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Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation

2016; Elsevier BV; Volume: 90; Linguagem: Inglês

10.1016/j.foodres.2016.10.036

1873-7145

Philippe E. Ramos, Luı́s Abrunhosa, Ana C. Pinheiro, Miguel A. Cerqueira, Carla Motta, Isabel Castanheira, Maria V. Chandra-Hioe, Jayashree Arcot, J. A. Teixeira, A. A. Vicente,

Child Nutrition and Feeding Issues

This study focused on the use of a new system, an alginate | Ɛ-poly-l-lysine | alginate | chitosan microcapsule (APACM), able to immobilize a folate-producing probiotic, Lactococcus lactis ssp. cremoris (LLC), which provides a new approach to the utilization of capsules and probiotics for in situ production of vitamins. LLC is able to produce 95.25 ± 26 μg·L− 1 of folate, during 10 h, and was encapsulated in the APACM. APACM proved its capacity to protect LLC against the harsh conditions of a simulated digestion maintaining a viable concentration of 6 log CFU·mL− 1of LLC. A nutrients exchange capacity test, was performed using Lactobacillus plantarum UM7, a high lactic acid producer was used here to avoid false negative results. The production and release of 2 g·L− 1 of lactic acid was achieved through encapsulation of L. plantarum, after 20 h. The adhesion of APACM to epithelial cells was also quantified, yielding 38% and 33% of capsules adhered to HT-29 cells and Caco-2 cells, respectively.