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Frequently asked questions about chlorophyll fluorescence, the sequel

2016; Springer Science+Business Media; Volume: 132; Issue: 1 Linguagem: Inglês

10.1007/s11120-016-0318-y

1573-5079

Hazem M. Kalaji, Gert Schansker, Marián Brestič, Filippo Bussotti, Ángeles Calatayud, Lorenzo Ferroni, Vasilij Goltsev, Lucia Guidi, Anjana Jajoo, Pengmin Li, Pasquale Losciale, Vinod Kumar Mishra, Amarendra Narayan Misra, Sergio G. Nebauer, Simonetta Pancaldi, Consuelo Penella, Martina Pollastrini, K. Suresh, Eduardo A. Tambussi, Marcos Yanniccari, Marek Živčák, Magdalena D. Cetner, Izabela A. Samborska, Alexandrina Stirbet, Katarína Olšovská, Kristyna Kunderlikova, Henry Shelonzek, Szymon Rusinowski, Wojciech Bąba,

Spectroscopy and Quantum Chemical Studies

Using chlorophyll (Chl) a fluorescence many aspects of the photosynthetic apparatus can be studied, both in vitro and, noninvasively, in vivo. Complementary techniques can help to interpret changes in the Chl a fluorescence kinetics. Kalaji et al. (Photosynth Res 122:121–158, 2014a) addressed several questions about instruments, methods and applications based on Chl a fluorescence. Here, additional Chl a fluorescence-related topics are discussed again in a question and answer format. Examples are the effect of connectivity on photochemical quenching, the correction of F V /F M values for PSI fluorescence, the energy partitioning concept, the interpretation of the complementary area, probing the donor side of PSII, the assignment of bands of 77 K fluorescence emission spectra to fluorescence emitters, the relationship between prompt and delayed fluorescence, potential problems when sampling tree canopies, the use of fluorescence parameters in QTL studies, the use of Chl a fluorescence in biosensor applications and the application of neural network approaches for the analysis of fluorescence measurements. The answers draw on knowledge from different Chl a fluorescence analysis domains, yielding in several cases new insights.