Dual-species transcriptional profiling during systemic candidiasis reveals organ-specific host-pathogen interactions
2016; Nature Portfolio; Volume: 6; Issue: 1 Linguagem: Inglês
10.1038/srep36055
ISSN2045-2322
AutoresBetty Hebecker, Sebastian Vlaic, Theresia Conrad, Michael Bauer, Sascha Brunke, Mario Kapitan, Jörg Linde, Bernhard Hube, Ilse D. Jacobsen,
Tópico(s)Tryptophan and brain disorders
ResumoAbstract Candida albicans is a common cause of life-threatening fungal bloodstream infections. In the murine model of systemic candidiasis, the kidney is the primary target organ while the fungal load declines over time in liver and spleen. To better understand these organ-specific differences in host-pathogen interaction, we performed gene expression profiling of murine kidney, liver and spleen and determined the fungal transcriptome in liver and kidney. We observed a delayed transcriptional immune response accompanied by late induction of fungal stress response genes in the kidneys. In contrast, early upregulation of the proinflammatory response in the liver was associated with a fungal transcriptome resembling response to phagocytosis, suggesting that phagocytes contribute significantly to fungal control in the liver. Notably, C. albicans hypha-associated genes were upregulated in the absence of visible filamentation in the liver, indicating an uncoupling of gene expression and morphology and a morphology-independent effect by hypha-associated genes in this organ. Consistently, integration of host and pathogen transcriptional data in an inter-species gene regulatory network indicated connections of C. albicans cell wall remodelling and metabolism to the organ-specific immune responses.
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