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Evaluation of 9 Rapid Agglutination Tests for the Diagnosis of Typhoid and Parathyphoid Fever in Côte D'ivoire

2016; Volume: 3; Issue: 1 Linguagem: Inglês

10.2174/1874220301603010243

1874-2203

André Inwoley, Roseline Affi-Aboli, Matthieu Kabran, Aimé Cézaire Adiko, Nadège-Audrey Abiyou, Franck Stéphane Amani, Jean-Claude Seka, Bamory Dembelé,

Vibrio bacteria research studies

Introduction: In Africa, the Widal test is done in the majority of laboratories by rapid tests whose performances are poorly documented. We evaluate the performance of 9 commercial rapid tests used in Côte d'Ivoire for the serodiagnosis of typhoid and paratyphoid fever. Methods: we used a panel of 170 samples collected from subjects suspected of typhoid or paratyphoid fever in Abidjan to determine the sensitivity, the specificity and operational characteristics of 9 plate agglutination tests (BIOREX ® , BIOTEC ® , CHRONOLAB ® , CROMATEST ® , FORTRESS ® , PLASMATEC ® , CYPRESS ® , SPINREACT ® and TYDAL ® ) compared to the reference technique SALMONELLA SEROLOGY ® of BIORAD (tube agglutination). Results: Among the 9 evaluated tests, only BIOTEC ® presented good performances (96% sensitivity and 97% specificity). Others gave sensitivities below 80%. Their characteristics were improved with the semi-quantitative plate agglutination test. All tests had good operational characteristics. Conclusion: In our context, the majority of tests evaluated should not be used to carry out the Widal test.