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Production and purification of amylolytic enzymes for saccharification of microalgal biomass

2016; Elsevier BV; Volume: 225; Linguagem: Inglês

10.1016/j.biortech.2016.11.047

1873-2976

Éllen Francine Rodrigues, A. M. M. FICANHA, Rogério Marcos Dallago, Helen Treichel, Christian Oliveira Reinehr, Tainara Paula Machado, Greice Borges Nunes, Luciane Maria Colla,

Biofuel production and bioconversion

The aim of this study was the production of amylolytic enzymes by solid state or submerged fermentations (SSF or SF, respectively), followed by purification using chemical process or microfiltration and immobilization of purified enzymes in a polyurethane support. The free and immobilized enzymes obtained were used to evaluate enzymatic hydrolysis of the polysaccharides of Spirulina. Microfiltration of the crude extracts resulted in an increase in their specific activity and thermal stability at 40 °C and 50 °C for 24 h, as compared to extracts obtained by SSF and SF. Immobilization of polyurethane purified enzyme produced yields of 332% and 205% for the enzymes obtained by SF and SSF, respectively. Free or immobilized enzymes favor the generation of fermentable sugar, being the application of the purified and immobilized enzymes in the hydrolysis of microalgal polysaccharides considered a promising alternative towards development of the bioethanol production process from microalgal biomass.