Produção Nacional
Revisado por pares
Parasitization of the Common Bean ( Phaseolus vulgaris ) by Meloidogyne ethiopica in Southern Brazil
2016; American Phytopathological Society; Volume: 101; Issue: 3 Linguagem: Inglês
10.1094/pdis-06-16-0841-pdn
ISSN1943-7692
AutoresCristiano Bellé, Paulo Roberto Kuhn, Tiago Edu Kaspary, Martin Groth, Juliane Schmitt, Stela Maris Kulczynski,
Tópico(s)Cassava research and cyanide
ResumoHomePlant DiseaseVol. 101, No. 3Parasitization of the Common Bean (Phaseolus vulgaris) by Meloidogyne ethiopica in Southern Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseParasitization of the Common Bean (Phaseolus vulgaris) by Meloidogyne ethiopica in Southern BrazilC. Bellé, P. R. Kuhn, T. E. Kaspary, M. Z. Groth, J. Schmitt, and S. M. KulczynskiC. Belléhttp://orcid.org/0000-0003-2247-3207Search for more papers by this author, P. R. KuhnSearch for more papers by this author, T. E. KasparySearch for more papers by this author, M. Z. GrothSearch for more papers by this author, J. SchmittSearch for more papers by this author, and S. M. KulczynskiSearch for more papers by this authorAffiliationsAuthors and Affiliations C. Bellé , Universidade Federal de Pelotas, 96010-900, Pelotas, RS, Brazil P. R. Kuhn , Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina, 89893-000, Mondaí, SC, Brazil T. E. Kaspary , Universidade Federal do Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil M. Z. Groth , Universidade Federal de Pelotas, 96010-900, Pelotas, RS, Brazil J. Schmitt S. M. Kulczynski , Universidade Federal de Santa Maria, Frederico Westphalen, 98400-000, RS, Brazil. Published Online:20 Dec 2016https://doi.org/10.1094/PDIS-06-16-0841-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Common bean (Phaseolus vulgaris L.) is currently a crop of great economic importance globally and occupies large areas of cultivation in Brazil with an average annual production of 3.5 million tons (MAPA 2016). Crop productivity has been limited by various phytosanitary diseases, with some caused by nematodes, in particular root-knot nematode (Meloidogyne spp.). During March 2014, common bean plants (cv. IPR Gralha) exhibiting symptoms of stunting, leaf yellowing, and numerous galls caused by root-knot nematode were detected and sampled in Caiçara County (27°16′ S; 53°27′ W), Northwest Rio Grande do Sul State, Brazil. The identification of Meloidogyne was conducted using esterase phenotypes (n = 20) (Carneiro and Almeida 2001), morphological analyses of females by perineal patterns (n = 20), morphological measurement of second-stage juveniles (J2) (n = 20), and amplification of the D2-D3 fragment of the 28S rRNA gene (primer set: D2A 5′-ACAAGTACCGTGAGGGAAAGTTG-3′ and D3B 5′-TCGGAAGGAACCAGCTACTA-3′) (De Ley et al. 1999). In addition, the root and soil samples were processed to determine the population density of nematode eggs and juveniles. The Meloidogyne population density was 425 eggs and J2 per gram of fresh roots and 234 J2 per 100 cm3 of soil. The esterase activity showed esterase pattern with three bands (Rm = 0.91, 1.05, and 1.23) corresponded to phenotype E3 of M. ethiopica (Carneiro et al. 2004). The perineal patterns of females with a moderately high-to-high dorsal arch showed smooth, widely spaced striae, similar to the description of M. ethiopica. The J2 had the following morphometric characteristics: length (L) = 485.75 ± 10.2 (320 to 510) µm, a = 25.1 ± 0.30 (21.5 to 28.0), c = 5.2 ± 0.86 (3.8 to 10.1), dorsal esophageal gland orifice (DGO) = 2.3 ± 0.65 (1.8 to 3.1) µm, stylet = 12.1 ± 0.9 (11.1 to 13.6) µm, tail length = 61.2 ± 0.84 μm (50.1 to 69.4) µm, and hyaline tail terminus = 13.1 ± 1.1 (12.3 to 14.8) µm. The DNA sequence of ∼750 bp fragment was obtained and deposited in the GenBank (accession no. KX377958). The sequence was compared with sequence availability in GenBank. The fragment showed 100% identity with the sequence of M. ethiopica isolates from Brazil (KF482372) and Chile (KF482373). The confirmation of nematode species identification was confirmed by PCR species-specific SCAR using a primer set meth-F (5′-ATGCAGCCGCAGGGAACGTAGTTG-3′) and meth-R (5′-GTTGTTTCATGTGCTTCGGCATC-3′). The PCR product was ∼350 bp for the SCAR, which was identical to previously reported for M. ethiopica (Correa et al. 2014). To confirm parasitization of common been by M. ethiopica, the greenhouse tests were conducted. Plantlets of common been cv. PR Gralha were maintained in eight pots containing sterilized soil and the pots were inoculated with 5,000 eggs/J2 of the M. ethiopica population. Noninoculated control was included in the test. After 60 days, all inoculated plants showed symptoms of chlorosis and reduced growth (reduction of approximately 36% compared with noninoculated control). Galling symptoms on the roots were similar to those observed in the field, and the nematode reproduction factor (RF = final population/initial population) was 18.9. The noninoculated plants did not present galls on the roots and their development was not affected. To our knowledge, this is the first report of M. ethiopica parasitizing common bean roots in Brazil. This finding is of great importance to Brazilian agriculture, since this nematode may damage common bean plants and become an additional problem for this crop.References:Carneiro, R. M. D. G., et al. 2004. Nematology 6:109. https://doi.org/10.1163/156854104323072982 Crossref, ISI, Google ScholarCarneiro, R. M. D. G., and Almeida, M. R. A. 2001. Nematol. Bras. 25:35. Google ScholarCorrea, V. R., et al. 2014. Plant Pathol. 63:476. https://doi.org/10.1111/ppa.12108 Crossref, ISI, Google ScholarDe Ley, P., et al. 1999. Nematology 1:591. https://doi.org/10.1163/156854199508559 Crossref, ISI, Google ScholarMAPA. 2016. Feijão, Ministério da Agricultura, Brasil. Retrieved from http://www.agricultura.gov.br/vegetal/culturas/feijao 30 May 2016. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 3 March 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 9 Feb 2017Published: 20 Dec 2016First Look: 14 Nov 2016Accepted: 8 Nov 2016 Pages: 510-510 Information© 2017 The American Phytopathological SocietyCited byMeloidogyne ethiopica (Root-knot nematode)CABI Compendium, Vol. CABI CompendiumHost weed species range of Meloidogyne ethiopica whitehead (Tylenchida: Meloidogynidae) found in Brazil25 November 2019 | European Journal of Plant Pathology, Vol. 156, No. 3Meloidogyne brasiliensis Charchar & Eisenback, 2002 is a junior synonym of M. ethiopica Whitehead, 1968Nematology, Vol. 19, No. 6
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