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First Report of a Resistance-Breaking Strain of Tomato spotted wilt virus Infecting Tomatoes With the Sw- 5 Tospovirus-Resistance Gene in California

2016; American Phytopathological Society; Volume: 101; Issue: 4 Linguagem: Inglês

10.1094/pdis-09-16-1371-pdn

1943-7692

Özgur Batuman, T. A. Turini, Paulo Oliveira, María R. Rojas, M. A. Macedo, H. Charles Mellinger, Scott Adkins, R. L. Gilbertson,

Plant-Microbe Interactions and Immunity

HomePlant DiseaseVol. 101, No. 4First Report of a Resistance-Breaking Strain of Tomato spotted wilt virus Infecting Tomatoes With the Sw-5 Tospovirus-Resistance Gene in California Previous DISEASE NOTES OPENOpen Access licenseFirst Report of a Resistance-Breaking Strain of Tomato spotted wilt virus Infecting Tomatoes With the Sw-5 Tospovirus-Resistance Gene in CaliforniaO. Batuman, T. A. Turini, P. V. Oliveira, M. R. Rojas, M. Macedo, H. C. Mellinger, S. Adkins, and R. L. GilbertsonO. Batuman, T. A. Turini, P. V. Oliveira, M. R. Rojas, M. Macedo, H. C. Mellinger, S. Adkins, and R. L. GilbertsonAffiliationsAuthors and Affiliations O. Batuman , University of Florida, Department of Plant Pathology, Southwest Florida Research and Education Center, Immokalee, FL 34142 T. A. Turini , University of California Agriculture and Natural Resources, Fresno County Cooperative Extension, Fresno, CA 93710 P. V. Oliveira , University of Cuiabá-Beira Rio, College of Agronomy, Cuiabá, MT, 78065 M. R. Rojas M. Macedo , Department of Plant Pathology, University of California-Davis, Davis, CA 95616 H. C. Mellinger , Glades Crop Care Inc., Jupiter, FL 33458 S. Adkins , USDA-ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945 R. L. Gilbertson , Department of Plant Pathology, University of California-Davis, Davis, CA 95616. Published Online:24 Jan 2017https://doi.org/10.1094/PDIS-09-16-1371-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Tomato spotted wilt virus (TSWV) is a highly destructive pathogen of fresh market and processing tomato (Solanum lycopersicum) in the Central Valley of California, U.S.A. In April 2016, severe tospovirus-like disease symptoms (i.e., stunting; leaf, stem, and petiole necrosis; and concentric rings on fruits) occurred in fields of fresh market tomato cultivars with the Sw-5 gene, which confers resistance to TSWV in Fresno County. Disease incidences of 30 to 50% were observed in some fields, and later reached 50 to 80%. Leaves with these symptoms were collected from four fresh market fields in Cantua Creek and one in Firebaugh, as well as from nearby processing tomato fields (n = 75). These plants were all positive for infection by TSWV when tested with AgDia immunostrips (Elkhart, IN), and RT-PCR with TSWV-specific N gene primers (Pappu et al. 1998). RT-PCR tests for Alfalfa mosaic, Tobacco/Tomato mosaic, Tomato chlorotic spot, and Groundnut ringspot viruses as well as ilar- and torradoviruses, were negative. Presence of the Sw-5 gene was confirmed by PCR with gene-specific primers (Dianese et al. 2010; Shi et al. 2011). To assess for resistance-breaking (RB) TSWV strains in these plants, RT-PCR and sequencing of the NSm was used to look for the amino acid substitutions, C118Y and T120N, previously associated with a Sw-5 RB TSWV strain in Spain (Lopez et al. 2011). The complete NSm gene was amplified from TSWV isolates in all 75 samples and each was directly sequenced in both directions. Sequence analysis showed that the complete NSm sequences were 99% identical to each other (e.g., KX898453 and KX898454), and revealed the C118Y but not the T120N substitution in the predicted NSm amino acid sequence from all TSWV-infected Sw-5 tomato plants tested (n = 45). Neither of these substitutions was present in the NSm of TSWV infecting processing non-Sw-5 tomato plants (n = 30). To assess infectivity, putative RB (C118Y substitution) isolates from Cantua Creek and Firebaugh and one wild-type isolate from Yolo County were mechanically inoculated onto three Sw-5 and one non-Sw-5 fresh market cultivars, and five Sw-5 and two non-Sw-5 processing tomato cultivars. In these experiments, the TSWV RB strains from California induced typical and severe symptoms of TSWV infection, similar to those observed in the field, 10 to 14 days post inoculation (dpi) in Sw-5 cultivars, whereas less severe symptoms appeared ∼30 dpi in a smaller number of plants of the non-Sw-5 cultivars. Disease incidence in the mechanically inoculated cultivars ranged from 10 to 100%. For the RB strains, RT-PCR and sequence analysis confirmed the presence of the C118Y substitution in the NSm of all isolates infecting Sw-5 cultivars and most (88%) infecting non-Sw-5 cultivars. None of the Sw-5 cultivars inoculated with wild-type TSWV developed disease symptoms by 30 dpi. PCR analysis confirmed presence/absence of Sw-5 in these cultivars as expected. Taken together, these results indicate that a TSWV RB strain has emerged in California, which can infect and cause typical symptoms in commercial fresh market and processing tomato cultivars with the Sw-5 gene. In field surveys, the TSWV RB strain was detected in Fresno but not in Yolo, Solano, or San Joaquin counties. This strain likely arose following a mutation, as Sw-5 cultivars have been widely grown in California over the past 5 years (e.g., ∼50% of processing cultivars grown in 2016).References:Dianese, E. C., et al. 2010. Mol. Breeding 25:133. Crossref, ISI, Google ScholarLopez, C., et al. 2011. J. Gen. Virol. 92:210. Crossref, ISI, Google ScholarPappu, H. R., et al. 1998. Virus Genes 17:169. Crossref, ISI, Google ScholarShi, A., et al. 2011. Am. J. Biotechnol. Mol. Sci. 1:8. https://doi.org/10.5251/ajbms.2011.1.1.8.16 Crossref, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 4 April 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Mar 2017Published: 24 Jan 2017First Look: 21 Nov 2016Accepted: 14 Nov 2016 Pages: 637-637 Information© 2017 The American Phytopathological SocietyCited byLeaf Curl Disease a Significant Constraint in the Production of Tomato: Impact, Challenges, and Management14 December 2022Viral Diseases of Tomato – Origins, Impact, and Future Prospects with a Focus on Tomato Spotted Wilt Virus and Tomato Yellow Leaf Curl Virus14 December 2022Biology and management of Sw-5 resistance-breaking Tomato spotted wilt virus isolates in Central CaliforniaActa Horticulturae, Vol. 2009, No. 1351Determination of key residues in tospoviral NSm required for Sw‐5b recognition, their potential ability to overcome resistance, and the effective resistance provided by improved Sw‐5b mutants27 December 2021 | Molecular Plant Pathology, Vol. 23, No. 5Tomato spotted wilt orthotospovirus (tomato spotted wilt)CABI Compendium, Vol. CABI CompendiumNovel Strategies for Management of Arthropod Vectors of Vector-borne Vegetable Diseases5 August 2022Pyramiding disease resistance in tomato by duplex PCR targeting resistance genes and exploiting gene linkage1 January 2022 | Crop Breeding and Applied Biotechnology, Vol. 22, No. 1Papaya Ring Spot Virus: An Understanding of a Severe Positive-Sense Single Stranded RNA Viral Disease and Its ManagementPhyton, Vol. 91, No. 10Stepwise artificial evolution of an Sw‐5b immune receptor extends its resistance spectrum against resistance‐breaking isolates of Tomato spotted wilt virus7 June 2021 | Plant Biotechnology Journal, Vol. 19, No. 11Resistance-breaking tomato spotted wilt orthotospovirus isolates on resistant tomato in Serbia17 June 2021 | Journal of Plant Diseases and Protection, Vol. 128, No. 5Natural Resources Resistance to Tomato Spotted Wilt Virus (TSWV) in Tomato (Solanum lycopersicum)12 October 2021 | International Journal of Molecular Sciences, Vol. 22, No. 20Discovery of Novel Chromone Derivatives as Potential Anti-TSWV Agents13 September 2021 | Journal of Agricultural and Food Chemistry, Vol. 69, No. 37Identification of new sources of resistance to resistance-breaking isolates of tomato spotted wilt virusSaudi Journal of Biological Sciences, Vol. 28, No. 5Induction of Plant Resistance in Tobacco (Nicotiana tabacum) against Tomato Spotted Wilt Orthotospovirus through Foliar Application of dsRNA12 April 2021 | Viruses, Vol. 13, No. 4Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors9 March 2021 | Pathogens, Vol. 10, No. 3Importance and epidemiology of tomato spotted wilt virusBiljni lekar, Vol. 49, No. 2Tomato Yellow Leaf Curl Virus-Resistant and -Susceptible Tomato Genotypes Similarly Impact the Virus Population Genetics7 December 2020 | Frontiers in Plant Science, Vol. 11Identification of Viruses and Viroids Infecting Tomato and Pepper Plants in Vietnam by Metatranscriptomics13 October 2020 | International Journal of Molecular Sciences, Vol. 21, No. 20A global invasion by the thrip, Frankliniella occidentalis : Current virus vector status and its management23 October 2019 | Insect Science, Vol. 27, No. 4Development of an IPM Strategy for Thrips and Tomato spotted wilt virus in Processing Tomatoes in the Central Valley of California5 August 2020 | Pathogens, Vol. 9, No. 8Application of a Reverse Genetic System for Beet Necrotic Yellow Vein Virus to Study Rz1 Resistance Response in Sugar Beet17 January 2020 | Frontiers in Plant Science, Vol. 10Comprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirus21 May 2019 | Scientific Reports, Vol. 9, No. 1A thrips vector of tomato spotted wilt virus responds to tomato acylsugar chemical diversity with reduced oviposition and virus inoculation20 November 2019 | Scientific Reports, Vol. 9, No. 1Biological and molecular characterization of tomato spotted wilt virus (TSWV) resistance‐breaking isolates from Argentina3 October 2019 | Plant Pathology, Vol. 68, No. 9Case study on processing tomatoesFirst Report of a Resistance-Breaking Strain of Tomato Spotted Wilt Orthotospovirus Infecting Sweet Pepper with the Tsw Resistance Gene in California, U.S.A.M. 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