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Sodium ascorbate kills Candida albicans in vitro via iron-catalyzed Fenton reaction: importance of oxygenation and metabolism

2016; Future Medicine; Volume: 11; Issue: 12 Linguagem: Inglês

10.2217/fmb-2016-0063

ISSN

1746-0921

Autores

Pinar Avci, Fernanda Freire, András Bánvölgyi, Eleftherios Mylonakis, Norbert Wikonkál, Michael R. Hamblin,

Tópico(s)

Medical and Biological Ozone Research

Resumo

Ascorbate can inhibit growth and even decrease viability of various microbial species including Candida albicans. However the optimum conditions and the mechanism of action are unclear. Materials/methodology: Candida albicans shaken for 90 min in a buffered solution of ascorbate (90 mM) gave a 5-log reduction of cell viability, while there was no killing without shaking, in growth media with different carbon sources or at 4°C. Killing was inhibited by the iron chelator 2,2'-bipyridyl. Hydroxyphenyl fluorescein probe showed the intracellular generation of hydroxyl radicals.Ascorbate-mediated killing of C. albicans depends on oxygenation and metabolism, involves iron-catalyzed generation of hydroxyl radicals via Fenton reaction and depletion of intracellular NADH. Ascorbate could serve as a component of a topical antifungal therapy.

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