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BIBTEX RIS

Differences between renal effects of venom from two Bothrops jararaca populations from southeastern and southern Brazil

2016; Elsevier BV; Volume: 125; Linguagem: Inglês

10.1016/j.toxicon.2016.11.249

1879-3150

Roberta Jeane Bezerra Jorge, Antônio Rafael Coelho Jorge, Ramon Róseo Paula Pessoa Bezerra de Menezes, Clarissa Perdigão Mello, Dânya Bandeira Lima, João Alison de Moraes Silveira, Natacha Teresa Queiroz Alves, Aline Diogo Marinho, Rafael Matos Ximenes, Carlos Corrêa-Netto, Larissa Gonçalves Machado, Russolina B. Zingali, Alice Maria Costa Martins, Helena Serra Azul Monteiro,

Healthcare and Venom Research

Components from animal venoms may vary according to the snake's age, gender and region of origin. Recently, we performed a proteomic analysis of Bothrops jararaca venom from southern (BjSv) and southeastern (BjSEv) Brazil, showing differences in the venom composition, as well as its biological activity. To continue the study, we report in this short communication the different effects induced by the BjSEv and BjSv on isolated kidney and MDCK renal cells. BjSEv decreased perfusion pressure (PP) and renal vascular resistance (RVR) and increased urinary flow (UF) and glomerular filtration rate (GFR), while BjSv did not alter PP and RVR and reduced UF and GFR. Both types of venom, more expressively BjSEv, reduced %TNa+, %TK+ and %Cl−. In MDCK cells, the two types of venom showed cytotoxicity with IC50 of 1.22 μg/mL for BjSv and 1.18 μg/mL for BjSEv and caused different profiles of cell death, with BjSv being more necrotic. In conclusion, we suggest that BjSv is more nephrotoxic than BjSEv.