Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Klebsiella pneumoniae Planktonic and Biofilm Reduction by Different Plant Extracts: In Vitro Study

2016; Hindawi Publishing Corporation; Volume: 2016; Linguagem: Inglês

10.1155/2016/3521413

2356-6140

Lucas de Paula Ramos, Carlos Eduardo da Rocha Santos, Daphne C. R. Mello, Lígia Nishiama Theodoro, Felipe Eduardo de Oliveira, Graziella Nuernberg Back‐Brito, Juliana Campos Junqueira, Antônio Olavo Cardoso Jorge, Luciane Dias de Oliveira,

Phytochemistry Medicinal Plant Applications

This study evaluated the action of Pfaffia paniculata K., Juglans regia L., and Rosmarius officinalis L. extracts against planktonic form and biofilm of Klebsiella pneumoniae (ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at 10 7 UFC/mL of K. pneumoniae was distributed into 96-well microplates ( n = 10 ) and after 48 h at 37°C and biofilm was subjected to treatment for 5 min with the extracts at a concentration of 200 mg/mL. ANOVA and Tukey tests (5%) were used to verify statistical significant reduction ( p < 0.05 ) of planktonic form and biofilm . P paniculata K., R. officinalis L., and J. regia L. showed reductions in biomass of 55.6, 58.1, and 18.65% and cell viability reduction of 72.4, 65.1, and 31.5%, respectively. The reduction obtained with P. paniculata and R. officinalis extracts was similar to the reduction obtained with chlorhexidine digluconate 2%. In conclusion, all extracts have microbicidal action on the planktonic form but only P. paniculata K. and R. officinalis L. were effective against biofilm.