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Precipitation of DNA with Ethanol

2016; Cold Spring Harbor Laboratory Press; Volume: 2016; Issue: 12 Linguagem: Inglês

10.1101/pdb.prot093377

1940-3402

Michael R. Green, Joseph Sambrook,

RNA modifications and cancer

DNA can be precipitated out of solution for the removal of salts and/or for resuspension in an alternative buffer. Either ethanol or isopropanol can be used to achieve this purpose; however, the use of ethanol is generally preferred. Cations, provided as salts, are typically included to neutralize the negative charge of the DNA phosphate backbone. This method describes ethanol precipitation of DNA in microcentrifuge tubes.