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Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure

2016; Elsevier BV; Volume: 158; Linguagem: Inglês

10.1016/j.carbpol.2016.11.088

1879-1344

Hui Zhang, Shaoping Nie, Steve W. Cui, Xu Ming, Huihuang Ding, Mingyong Xie,

Mycorrhizal Fungi and Plant Interactions

The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC–MS analysis, and 1D & 2D NMR spectroscopy. Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%). Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1 → 3)-glucan. The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2. This revised structure as an acidic β-(1 → 3, 1 → 6)-glucan is considered to be more accurate than the previous proposal of PSG-1.