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BIBTEX RIS

Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens

2016; Cambridge University Press; Volume: 144; Issue: 2 Linguagem: Inglês

10.1017/s0031182016001645

1469-8161

Marcelo Andreetta Corral, Fabiana Martins de Paula, Dirce Mary Correa Meisel, Vera Lúcia Pagliusi Castilho, EMN Gonçalves, David A. Levy, Sérgio Paulo Bydlowski, Pedro Paulo Chieffi, William Castro‐Borges, Ronaldo Cesar Borges Gryschek,

Parasitic Diseases Research and Treatment

SUMMARY Strongyloides venezuelensis is a parasitic nematode of rodents that is frequently used to obtain heterologous antigens for immunological diagnosis of human strongyloidiasis. The aim of this study was to identify antigens from filariform larvae of S. venezuelensis for immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from filariform larvae of S. venezuelensis were obtained in phosphate saline (SS and SM) and in Tris–HCl buffer (TS and TM), and were analysed by Western blotting. Different antigenic components were recognized by IgG antibodies from the sera of strongyloidiasis patients. Highest recognition was observed for a 30–40 kDa mass range present in all antigenic fractions. The band encompassing this mass range was then excised and subjected to mass spectrometry for protein identification. Immunoreactive proteins identified in the soluble fractions corresponded to metabolic enzymes, whereas cytoskeletal proteins and galectins were more abundant in the membrane fractions. These results represent the first approach towards identification of S. venezuelensis antigens for use in immunodiagnostic assays for human strongyloidiasis.