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A multiplex PCR approach for the molecular identification and conservation of the Critically Endangered daggernose shark

2016; Inter-Research; Volume: 32; Linguagem: Inglês

10.3354/esr00798

1863-5407

Pedro G. Nachtigall, LFS Rodrigues-Filho, Davidson Sodré, Marcelo Vallinoto, Danillo Pinhal,

Fish biology, ecology, and behavior

ESR Endangered Species Research Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials ESR 32:169-175 (2017) - DOI: https://doi.org/10.3354/esr00798 A multiplex PCR approach for the molecular identification and conservation of the Critically Endangered daggernose shark Pedro G. Nachtigall1*, Luis F. S. Rodrigues-Filho2, Davidson C. A. Sodré2, Marcelo Vallinoto2, Danillo Pinhal1 1Departamento de Genética, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, SP 18618-970, Brazil 2Instituto de Estudos Costeiros (IECOS), Universidade Federal do Pará - UFPA, Bragança, PA, Brazil *Corresponding author: pedronachtigall@gmail.com ABSTRACT: The daggernose shark Isogomphodon oxyrhynchus is an inshore tropical species endemic to the coastal waters off northeastern South America with limited distribution and restrictive biological traits. According to the IUCN, this species is Critically Endangered owing to a dramatic population decline over the past 10 yr. Here we implemented a multiplex PCR methodology based on species-specific primers for the precise identification of samples from the daggernose shark (body parts and fins). The effectiveness of the method was verified through application for analysis of unidentified shark samples collected in fish markets and a worldwide inventory of over 57 shark species. This reliable molecular system of identification combines the advantages of low cost and high throughput and may be used locally to monitor the capture and trade of this Critically Endangered shark species. KEY WORDS: Genetic identification · ITS2 · Multiplex PCR · Sharks · DNA forensics Full text in pdf format Supplementary material PreviousNextCite this article as: Nachtigall PG, Rodrigues-Filho LFS, Sodré DCA, Vallinoto M, Pinhal D (2017) A multiplex PCR approach for the molecular identification and conservation of the Critically Endangered daggernose shark. Endang Species Res 32:169-175. https://doi.org/10.3354/esr00798 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in ESR Vol. 32. Online publication date: February 23, 2017 Print ISSN: 1863-5407; Online ISSN: 1613-4796 Copyright © 2017 Inter-Research.