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Reductive carboxylation is a major metabolic pathway in the retinal pigment epithelium

2016; National Academy of Sciences; Volume: 113; Issue: 51 Linguagem: Inglês

10.1073/pnas.1604572113

1091-6490

Jianhai Du, Aya Yanagida, Kaitlen Knight, Abbi L. Engel, Anh Huan Vo, Connor S.R. Jankowski, Martin Sadı́lek, Van Tran, Megan A. Manson, Aravind Ramakrishnan, James B. Hurley, Jennifer R. Chao,

Retinoids in leukemia and cellular processes

Significance In the vertebrate eye, a monolayer of cells, called the retinal pigment epithelium (RPE), is between the choroidal blood supply and the retina. The RPE provides metabolic support for the retina, including delivery of glucose and other nutrients. Here, we show that reductive carboxylation of α-ketoglutarate, a type of metabolism that supports growth and survival of cancer cells, is a prominent feature of RPE cells. We show that extreme oxidative stress can overwhelm the reductive carboxylation pathway. However, we also found that the RPE can be protected from extreme oxidative stress by supplementation with an NAD + precursor or α-ketoglutarate.