Spectrophotometric Detenmination of Spermine with Vanillylfluorone and Cobalt(II)
2016; Japan Society for Analytical Chemistry; Volume: 65; Issue: 11 Linguagem: Inglês
10.2116/bunsekikagaku.65.671
ISSN0525-1931
AutoresShowa KASHIWAGI, Momoka Matsushita, Kanako Miyachi, Mamiko Asano, Mihoyo Fujitake, Takako Yamaguchi, Hitoshi Matsumura, Yoshikazu Fujita,
Tópico(s)Protein Hydrolysis and Bioactive Peptides
ResumoA novel spectrophotometric method was established for the determination of spermine based on color complex formation among vanillylfluorone, cobalt(II) and spermine in the presence of stearyltrimethylammonium chloride. In the determination of spermine, Beer's low was obeyed in the range of 0.04 - 1.0 μg mL−1. The effective molar absorptivity at 615 nm and the relative standard deviation were 2.2 × 105 L mol−1 cm−1 and 2.5 % (n = 5), respectively.
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