Produção Nacional
Revisado por pares
First Report of Root Rot caused by Rosellinia bunodes on a Poplar Species ( Populus deltoides ) in Brazil
2016; American Phytopathological Society; Volume: 101; Issue: 4 Linguagem: Inglês
10.1094/pdis-09-16-1268-pdn
ISSN1943-7692
AutoresA. F. dos Santos, Heloisa Thomazi, Henrique da Silva Silveira Duarte, Edilene Buturi Machado, C. N. Silva, Dauri José Tessmann,
Tópico(s)Plant Pathogens and Fungal Diseases
ResumoHomePlant DiseaseVol. 101, No. 4First Report of Root Rot caused by Rosellinia bunodes on a Poplar Species (Populus deltoides) in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Root Rot caused by Rosellinia bunodes on a Poplar Species (Populus deltoides) in BrazilA. F. dos Santos, H. Thomazi, H. S. S. Duarte, E. B. Machado, C. N. Silva, and D. J. TessmannA. F. dos SantosSearch for more papers by this author, H. ThomaziSearch for more papers by this author, H. S. S. DuarteSearch for more papers by this author, E. B. MachadoSearch for more papers by this author, C. N. SilvaSearch for more papers by this author, and D. J. TessmannSearch for more papers by this authorAffiliationsAuthors and Affiliations A. F. dos Santos , Empresa Brasileira de Pesquisa Agropecuária – Embrapa Forestry, Colombo 83411-000, Paraná, Brazil H. Thomazi H. S. S. Duarte , Universidade Federal do Paraná, Curitiba 80035-060, Paraná, Brazil E. B. Machado , Swedish Match, Curitiba 80230-180, Paraná, Brazil C. N. Silva D. J. Tessmann , Universidade Estadual de Maringá, Maringá 87020-090, Paraná, Brazil. Published Online:8 Feb 2017https://doi.org/10.1094/PDIS-09-16-1268-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Populus deltoides Bartr. ex Marsh., also known as eastern cottonwood, is a forest species native to North America that was introduced in southern Brazil for wood production in the 1990s. Since 2010, poplar trees over 2 years old have been found in commercial plantations exhibiting leaf yellowing followed by leaf fall with severe root rot. Dead poplars have been found amid scattered patches of diseased trees. Fungal cultures consistently isolated from roots of diseased trees were purified by using the hyphal tip method (Dhingra and Sinclair 1985). Cultures did not produce any specialized structures associated with sexual or asexual reproduction. However, they presented the typical pear-shaped swelling at the septa of mycelia, which is characteristic of the genus Rosellinia (Castro et al. 2013). DNA was extracted from two cultures and used for amplification of the ITS-5.8S rDNA region by PCR using the primers ITS4 and ITS5 described by White et al. (1990). The PCR products (530 bp) were sequenced and compared in GenBank using BLAST analysis. The sequences (KU935438 and KU935439) were 99% similar with Rosellinia bunodes (Berk. & Broome) Sacc., accession no. AB609598.1 (Castro et al. 2013). To confirm pathogenicity, four isolates of R. bunodes were used to inoculate twelve 6-month-old poplar seedlings. Sorghum grains colonized by R. bunodes were used as inoculum. For inoculum production, seven 8 mm diameter mycelial plugs taken from the edge of 12-day-old potato dextrose agar (PDA) cultures, incubated at 24 ± 2°C in the dark, were transferred to 250 ml Erlenmeyer flasks containing 80 g of previously autoclaved sorghum grains. After 17 days of incubation at 24 ± 2°C in the dark, grains colonized by the fungus were mixed with a commercial plant substrate, in the proportion of 1 kg of substrate to 32 g of sorghum grains. The mixture was placed in plastic pots where poplar seedlings were transplanted. The control plants were transplanted to a substrate containing uncolonized grains. The fungus reisolated on PDA plates from infected seedlings was morphologically identical to field isolates. Thirteen days after transplantation, dead plants were already found, proving the pathogenicity of the fungal isolates. No symptoms were observed on control plants. It is interesting to note that our isolate also caused symptoms on araucaria pine (Araucaria angustifolia) and yerba mate (Ilex paraguaiensis) seedlings, two Brazilian native forest species, and that R. bunodes was reisolated from the inoculated seedlings. No symptoms occurred on the control plants. Live cultures of R. bunodes are preserved at the Laboratory of Forest Pathology of Embrapa Forestry, Colombo, Paraná, Brazil. To our knowledge, this is the first report of R. bunodes causing root rot on poplar in Brazil.References:Castro, B. L., et al. 2013. Australas. Plant Pathol. 42:515. https://doi.org/10.1007/s13313-013-0205-3 Crossref, ISI, Google ScholarDhingra, O. D., and Sinclair, J. B. 1985. Basic Plant Pathology Methods. CRC Press, Boca Raton, FL. Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego. Crossref, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 4 April 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Mar 2017Published: 8 Feb 2017First Look: 5 Dec 2016Accepted: 28 Nov 2016 Page: 632 Information© 2017 The American Phytopathological SocietyCited byPathogens of the Araucariaceae: How Much Do We Know?23 May 2022 | Current Forestry Reports, Vol. 8, No. 2Rosellinia bunodes (black root rot)CABI Compendium, Vol. CABI CompendiumOne stop shop III: taxonomic update with molecular phylogeny for important phytopathogenic genera: 51–75 (2019)17 September 2019 | Fungal Diversity, Vol. 98, No. 1
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