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UHPLC–Q‐TOF‐MS‐based metabolomics approach to compare the saponin compositions of Xueshuantong injection and Xuesaitong injection

2016; Wiley; Volume: 40; Issue: 4 Linguagem: Inglês

10.1002/jssc.201601122

1615-9314

Changliang Yao, Wenzhi Yang, Jingxian Zhang, Shi Qiu, Ming Chen, Xiaojian Shi, Huiqin Pan, Wanying Wu, De‐an Guo,

Traditional Chinese Medicine Analysis

Various traditional Chinese medicine preparations developed from Notoginseng total saponins, including Xueshuantong injection and Xuesaitong injection, are extensively used in China to treat cardiocerebrovascular diseases. However, the difference of their saponin compositions remains unknown. An ultra high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry based metabolomics approach was developed to probe the saponin discrimination between Xueshuantong and Xuesaitong and the related factors by large sample analysis. A highly efficient chromatographic separation was achieved on an HSS T3 column within 20 min with the holistic metabolites information recorded in the negative MS E mode. A six‐step data pretreatment procedure mainly based on Progenesis QI and mass defect filtering was established. Pattern recognition chemometrics was used to discover the potential saponin markers. The saponin composition of Wuzhou Xueshuantong showed distinct discrimination from the other products. Wuzhou Xueshuantong contains more abundant protopanaxatriol‐type noto‐R 1 , Rg 1 , Re, and protopanaxadiol‐type Rb 1 , but less Rd and other low‐polarity protopanaxadiol‐type ginsenosides. These differences could not directly correlate to the use of different parts of Panax notoginseng , but possibly to the different preparation techniques employed by different manufacturers. These results are beneficial to the establishment of pharmacopoeia standards and the assessment of the efficacy and adverse drug reactions for these homologous products.