Produção Nacional
Revisado por pares
Amaranthus viridis is a Weed Host of Meloidogyne arenaria in Rio Grande do Sul State, Brazil
2016; American Phytopathological Society; Volume: 101; Issue: 4 Linguagem: Inglês
10.1094/pdis-10-16-1428-pdn
ISSN1943-7692
AutoresTiago Edu Kaspary, Cristiano Bellé, Martin Groth, Kassia Luiza Teixeira Cocco, Luan Cutti, Gabriele Casarotto,
Tópico(s)Cassava research and cyanide
ResumoHomePlant DiseaseVol. 101, No. 4Amaranthus viridis is a Weed Host of Meloidogyne arenaria in Rio Grande do Sul State, Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseAmaranthus viridis is a Weed Host of Meloidogyne arenaria in Rio Grande do Sul State, BrazilT. E. Kaspary, C. Bellé, M. Z. Groth, K. L. T. Cocco, L. Cutti, and G. CasarottoT. E. Kaspary, C. Belléhttp://orcid.org/0000-0003-2247-3207, M. Z. Groth, K. L. T. Cocco, L. Cutti, and G. CasarottoAffiliationsAuthors and Affiliations T. E. Kaspary , Universidade Federal do Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil C. Bellé M. Z. Groth K. L. T. Cocco , Universidade Federal de Pelotas, 96010-900, Pelotas, RS, Brazil L. Cutti G. Casarotto , Universidade Federal do Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil. Published Online:3 Feb 2017https://doi.org/10.1094/PDIS-10-16-1428-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Weeds reduce agricultural productivity by competing for environmental resources and serving as alternative hosts for pests and pathogens. Root-knot nematodes (Meloidogyne spp.) have been reported parasitize various species of weeds, including Amaranthus spp., which are widely distributed in Brazil (Kokalis-Burelle and Rosskopf 2012). During the summer of 2015, specimens of slender amaranth (Amaranthus viridis L.) with multiple root galls were detected in a soybean production field (27°53′S, 53°04′W) from Novo Barreiro, Rio Grande do Sul, Brazil. Root and soil samples were processed. The Meloidogyne population density was 702 eggs and second-stage juveniles (J2) per gram of fresh root and 345 J2 per 100 cm3 of soil. The Meloidogyne J2 morphometric characters included: body length = 473.21 ± 18.21 µm; body width = 15.13 ± 0.80 µm; a = 31.20 ± 2.3; c = 8.45 ± 0.77; DGO from stylet base = 3.01 ± 0.36 µm; stylet = 13.01 ± 1.12 µm; tail length = 55.10 ± 5.00 µm; hyaline tail terminus = 9.50 ± 0.81 µm; matching the description of M. arenaria (Cliff and Hirschmann 1985). Female nematodes (n = 20) were dissected from the respective root samples and subjected to esterase isoenzyme electrophoresis (Carneiro and Almeida 2001), morphological analysis of perineal patterns, and amplification of the mitochondrial DNA region between COII and 16S using the primers C2F3/1108. Electrophoresis revealed the polymorphic esterase bands of the phenotype A2 (Rm = 1.25, 1.35) typical of M. arenaria (Carneiro et al. 2008). Perineal patterns exhibited a low dorsal arch, with lateral field marked by forked and broken striae similar to those described in M. arenaria. The PCR amplified product (accession no. KX280643) was sequenced and aligned with sequences obtained from GenBank. BLAST analysis resulted in a 100% similarity to the sequences of M. arenaria previously isolated from Brazil (AY635610), China (JQ446377), Costa Rica (KF993637), and South Africa (KC287201 and KC287200). Further verification came from the species-specific SCAR primer set Far/Rar, which produced a 420-bp fragment identical (same size) to the fragment previously reported for M. arenaria (Zijlstra et al. 2000). To confirm nematode pathogenicity, plantlets of A. viridis were maintained in six pots containing sterilized soil and the pots were inoculated with 5,000 eggs plus J2s of the original population of M. arenaria. Noninoculated plants were used as controls (six replicates). After 70 days, inoculated plants showed root galling, similar to the original plants encountered in the field and the mean nematode reproduction factor (final population/initial population) was 32.7. The noninoculated plants did not present galls in the roots. This positive identification indicates that A. viridis serves as an important host for M. arenaria, making control of this weed essential for managing the nematode. To our knowledge, this is the first formal record of M. arenaria parasitizing A. viridis in Brazil.References:Carneiro, R. M. D. G., et al. 2008. Nematology 10:819. https://doi.org/10.1163/156854108786161526 Crossref, ISI, Google ScholarCarneiro, R. M. D. G., and Almeida, M. R. A. 2001. Nematol. Bras. 25:35. Google ScholarCliff, G., and Hirschmann, H. 1985. J. Nematol. 17:445. ISI, Google ScholarKokalis-Burelle, N., and Rosskopf, E. N. 2012. J. Nematol. 44:142. ISI, Google ScholarZijlstra, C., et al. 2000. Nematology 2:847. https://doi.org/10.1163/156854108786161526 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 4 April 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Mar 2017Published: 3 Feb 2017First Look: 5 Dec 2016Accepted: 28 Nov 2016 Page: 635 Information© 2017 The American Phytopathological SocietyCited byAmaranthus viridis (slender amaranth)CABI Compendium, Vol. CABI CompendiumMeloidogyne arenaria (peanut root-knot nematode)CABI Compendium, Vol. CABI CompendiumHost status of morning-glory ( Ipomoea spp.) to Meloidogyne species1 January 2021 | Journal of Nematology, Vol. 53, No. 1Multiplication potential of Meloidogyne arenaria in weeds found in Brazil8 May 2020 | European Journal of Plant Pathology, Vol. 157, No. 2Reproduction of Meloidogyne enterolobii on weeds found in Brazil28 February 2019 | Tropical Plant Pathology, Vol. 44, No. 4Facile synthesis of anhydrojudaicin and 11,13-dehydroanhydrojudaicin, two eudesmanolide-skeleton lactones with potential allelopathic activityPhytochemistry Letters, Vol. 31Meloidogyne Species Associated with Weeds in Rio Grande do Sul1 January 2019 | Planta Daninha, Vol. 37Leaf Consumption and Preference to Conyza sp., Conventional and Bt Soybean by Helicoverpa armigera1 January 2019 | Planta Daninha, Vol. 37
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