Artigo Acesso aberto Produção Nacional Revisado por pares

Morphophysiological characterization of rhizobia isolated from wild genotypes of the common bean

2016; UNIVERSIDADE FEDERAL DE UBERLÂNDIA; Linguagem: Inglês

10.14393/bj-v32n6a2016-33084

ISSN

1981-3163

Autores

Fernanda Bueno Sampaio, A. M. Knupp, Eliana Paula Fernandes, Claudia Cristina Garcia Martin-Didonet, Enderson Petrônio de Brito Ferreira,

Tópico(s)

Soybean genetics and cultivation

Resumo

This study aimed to characterize, based on the morphology and on the use of carbon sources, rhizobial isolates obtained from nodules of wild common bean genotypes cultivated on soil samples from the states of Goiás, Minas Gerais and Paraná.Based on morphological and carbon source use, similarity matrixes were generated to perform clustering analysis based on the Jaccard index.A total of 523 isolates were obtained with the predominance of isolates that acidify the culture medium and showed fast growth.Isolates from the soils of Araucária and Prudentópolis produced a greater amount of mucus than those from Jussara, Nova Veneza, Uberlândia and Unaí soils.The rhizobia isolates obtained from soils of Goiás and Minas Gerais showed greater phenotypic diversity than those from the soils of Paraná.Analysis based on the use of different carbon sources revealed that rhizobia isolates obtained from soils of Goiás have higher metabolic diversity.Overall, the most part of isolates grouped with the reference strains, especially with the Rhizobium tropici and R. freirei strains used as inoculants for the common bean.

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