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BIBTEX RIS

18-Crown-6 spiking in direct infusion ESI–MS analysis of complex mixtures: “One ion per analyte” relationship facilitating ion assignments and eliminating isobaric interferences

2016; Elsevier BV; Volume: 418; Linguagem: Inglês

10.1016/j.ijms.2016.12.003

ISSN

1873-2798

Autores

Angélica Priscila Parussolo Tonin, Marcos Ribeiro, Valquíria de Moraes Silva, Cláudio C. Oliveira, M.H. Saragiotto, Marcos N. Eberlin, Eduardo César Meurer,

Tópico(s)

Metabolomics and Mass Spectrometry Studies

Resumo

Spiking with 18-crown-6/trifluoracetic acid is shown to sequester metal cations thus eliminating unwanted [TAG+Na]+ and [TAG+K]+ ions whereas favoring analyte detection as sole [TAG+H]+ ions in the ESI(+)-MS analysis of methanolic solutions of soybean oil. This sequestering eliminates ion splitting and its resulting isobaric interferences, thus increasing spectra quality greatly facilitates analyte identification. This "one ion per analyte relationship" is highly beneficial for direct infusion analysis of complex mixtures by ESI–MS.

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