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Selective Visualization of the Endogenous Peroxynitrite in an Inflamed Mouse Model by a Mitochondria-Targetable Two-Photon Ratiometric Fluorescent Probe

2016; American Chemical Society; Volume: 139; Issue: 1 Linguagem: Inglês

10.1021/jacs.6b10508

1943-2984

Dan Cheng, Yue Pan, Lu Wang, Zebing Zeng, Lin Yuan, Xiaobing Zhang, Young‐Tae Chang,

Sulfur Compounds in Biology

Peroxynitrite (ONOO–) is a kind of reactive oxygen species (ROS) with super activity of oxidization and nitration, and overproduction of ONOO– is associated with pathogenesis of many diseases. Thus, accurate detection of ONOO– with high sensitivity and selectivity is imperative for elucidating its functions in health or disease states. Herein we for the first time present a new two-photon ratiometric fluorescent ONOO– probe (MITO-CC) based on FRET mechanism by combining rational design strategy and dye-screening approach. MITO-CC, with fast response rate (within 20 s), excellent sensitivity (detection limit = 11.30 nM) and outstanding selectivity toward ONOO–, was successfully applied to ratiometric detection of endogenous ONOO– produced by HepG2/RAW264.7 cells and further employed for imaging oxidative stress in an inflamed mouse model. Therefore, probe MITO-CC could be a potential biological tool to explore the roles of ONOO– under different physiological and pathological settings.