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Sandwich-Type Enzymatic Fuel Cell Based on a New Electro-Conductive Material - Ion Jelly

2016; Wiley; Volume: 1; Issue: 20 Linguagem: Inglês

10.1002/slct.201601640

2365-6549

Rui N. L. Carvalho, Rui M. Almeida, José J. G. Moura, Nuno T. Lourenço, Luís P. Fonseca, Cristina M. Cordas,

Electrocatalysts for Energy Conversion

ChemistrySelectVolume 1, Issue 20 p. 6546-6552 Full Paper Sandwich-Type Enzymatic Fuel Cell Based on a New Electro-Conductive Material - Ion Jelly Rui N. L. Carvalho, Rui N. L. Carvalho iBB – Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisbon, PortugalSearch for more papers by this authorDr. Rui M. Almeida, Dr. Rui M. Almeida UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciência e Tecnologia, Universidade Nova de Lisboa, 2819-516 Caparica, PortugalSearch for more papers by this authorProf. José J. G. Moura, Prof. José J. G. Moura UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciência e Tecnologia, Universidade Nova de Lisboa, 2819-516 Caparica, PortugalSearch for more papers by this authorDr. Nuno T. Lourenço, Dr. Nuno T. Lourenço iBB – Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisbon, PortugalSearch for more papers by this authorProf. Luís J. P. Fonseca, Prof. Luís J. P. Fonseca iBB – Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisbon, PortugalSearch for more papers by this authorDr. Cristina M. Cordas, Corresponding Author Dr. Cristina M. Cordas c.cordas@fct.unl.pt UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciência e Tecnologia, Universidade Nova de Lisboa, 2819-516 Caparica, PortugalSearch for more papers by this author Rui N. L. Carvalho, Rui N. L. Carvalho iBB – Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisbon, PortugalSearch for more papers by this authorDr. Rui M. Almeida, Dr. Rui M. Almeida UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciência e Tecnologia, Universidade Nova de Lisboa, 2819-516 Caparica, PortugalSearch for more papers by this authorProf. José J. G. Moura, Prof. José J. G. Moura UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciência e Tecnologia, Universidade Nova de Lisboa, 2819-516 Caparica, PortugalSearch for more papers by this authorDr. Nuno T. Lourenço, Dr. Nuno T. Lourenço iBB – Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisbon, PortugalSearch for more papers by this authorProf. Luís J. P. Fonseca, Prof. Luís J. P. Fonseca iBB – Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisbon, PortugalSearch for more papers by this authorDr. Cristina M. Cordas, Corresponding Author Dr. Cristina M. Cordas c.cordas@fct.unl.pt UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciência e Tecnologia, Universidade Nova de Lisboa, 2819-516 Caparica, PortugalSearch for more papers by this author First published: 15 December 2016 https://doi.org/10.1002/slct.201601640Citations: 4Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Graphical Abstract Redox enzymes were immobilized in a conductive gelatin-ionic liquid hybrid material, Ion Jelly (IJ). Layers of IJ with cytochrome c3, hydrogenase and aldehyde oxidoreductase were deposited on screen printed electrodes (SPEs) and characterized electrochemically. Cell-type configurations were constructed by depositing up to four IJ layers sandwiched between two SPEs (see picture), establishing an electronic transport chain between the enzymes Abstract In the present work, Ion Jelly films based on 1-ethyl-3-methylimidazolium ethylsulfate, with incorporated redox proteins and enzymes were deposited on carbon screen-printed electrodes (SPEs), and their electrochemical characterization was attained. Ion Jelly synthesis was carried out simultaneously with protein incorporation in its structure, followed by a maturation step under controlled atmosphere (4 days at 4 °C and water activity (aw) of 0.76). The electrochemical response of the material was characterized, and a sandwich-type fuel cell configuration was subsequently built, consisting of two SPEs containing two independent Ion Jelly discs in the middle; one disc incorporated Desulfovibrio gigas cytochrome c3 and [NiFe]-hydrogenase, while the other disc contained aldehyde oxidoreductase, constituting the biocathode and bioanode of the cell, respectively. Cell voltage increased with time in the presence of benzaldehyde, in agreement with a successful electronic pathway across the cell and the concomitant aldehyde oxidoreductase enzymatic activity. In the cathodic side assays, hydrogenase showed catalytic activity towards H+ reduction to H2. Conflict of interest The authors declare no conflict of interest. Citing Literature Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description slct201601640-sup-0001-misc_information.pdf547.4 KB Supplementary Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume1, Issue20December 1, 2016Pages 6546-6552 RelatedInformation