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One-step isolation of carnosic acid and carnosol from rosemary by centrifugal partition chromatography

2016; Wiley; Volume: 40; Issue: 5 Linguagem: Inglês

10.1002/jssc.201601063

1615-9314

Mary H. Grace, Yin Qiang, Shengmin Sang, Mary Ann Lila,

Phytochemicals and Antioxidant Activities

Journal of Separation ScienceVolume 40, Issue 5 p. 1057-1062 RESEARCH ARTICLE One-step isolation of carnosic acid and carnosol from rosemary by centrifugal partition chromatography Mary H. Grace, Mary H. Grace Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, NC, USASearch for more papers by this authorYin Qiang, Yin Qiang Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, NC, USA School of Pharmacy, Lanzhou University, Lanzhou, P.R. ChinaSearch for more papers by this authorShengmin Sang, Shengmin Sang Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, Kannapolis, NC, USASearch for more papers by this authorMary Ann Lila, Corresponding Author Mary Ann Lila mlila@ncsu.edu Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, NC, USA Correspondence Dr. Mary Ann Lila, Director, Plants for Human Health Institute, 600 Laureate Way, Kannapolis, NC 28081, USA. Email: mlila@ncsu.edu Fax: 704-250-5409Search for more papers by this author Mary H. Grace, Mary H. Grace Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, NC, USASearch for more papers by this authorYin Qiang, Yin Qiang Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, NC, USA School of Pharmacy, Lanzhou University, Lanzhou, P.R. ChinaSearch for more papers by this authorShengmin Sang, Shengmin Sang Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, Kannapolis, NC, USASearch for more papers by this authorMary Ann Lila, Corresponding Author Mary Ann Lila mlila@ncsu.edu Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, NC, USA Correspondence Dr. Mary Ann Lila, Director, Plants for Human Health Institute, 600 Laureate Way, Kannapolis, NC 28081, USA. Email: mlila@ncsu.edu Fax: 704-250-5409Search for more papers by this author First published: 23 December 2016 https://doi.org/10.1002/jssc.201601063Citations: 12 Conflict of interest: The authors have declared no conflict of interest. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Carnosic acid and carnosol are the main bioactive components responsible for the significant antioxidant activity of Rosmarinus officinalis. Nevertheless, they are known for their instability in solutions. Separation of both compounds from crude rosemary extract was successfully achieved by one-step centrifugal partition chromatography without any degradation. A two-phase solvent system, hexane/ethyl acetate/methanol/water (3:2:3:2 v/v) was run on a preparative scale applying the elution–extrusion technique in descending mode. A 900 mg quantity of the crude extract containing 39.7% carnosic acid and 12.3% carnosol was loaded onto a 500 mL column, rotating at 1800 rpm. Carnosic acid and carnosol were obtained at purities of 96.1 ± 1% and 94.4 ± 0.9%, with recoveries of 94.3 ± 4.4% and 94.8 ± 2.3%, respectively. The compounds were identified by mass spectrometry, tandem mass spectrometry, and comparison with authentic standards. Citing Literature Supporting Information Filename Description jssc5269-sup-0001-SuppMat.docx43.9 KB Table S1. LC-IT-TOF-ESI-MS and MS/MS of compounds detected in rosemary extract in the negative ion mode. Figure 1S. Standard curve of carnosic acid authentic reference Figure 2S. Standard curve for carnosol authentic reference Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume40, Issue5March 2017Pages 1057-1062 RelatedInformation