Neutrophil extracellular traps in acute chorioamnionitis: A mechanism of host defense
2017; Wiley; Volume: 77; Issue: 3 Linguagem: Inglês
10.1111/aji.12617
ISSN1600-0897
AutoresNardhy Gomez‐Lopez, Roberto Romero, Yaozhu Leng, Valeria Garcia‐Flores, Yi Xu, Derek Miller, Sonia S. Hassan,
Tópico(s)Neonatal Respiratory Health Research
ResumoAmerican Journal of Reproductive ImmunologyVolume 77, Issue 3 e12617 ORIGINAL ARTICLE Neutrophil extracellular traps in acute chorioamnionitis: A mechanism of host defense Nardhy Gomez-Lopez, Corresponding Author Nardhy Gomez-Lopez ngomezlo@med.wayne.edu Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI, USA Correspondence Nardhy Gomez-Lopez, Perinatology Research Branch, NICHD/NIH/DHHS, Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. Email: ngomezlo@med.wayne.edu and Roberto Romero, Perinatology Research Branch, NICHD/NIH/DHHS, Hutzel Women's Hospital, Detroit, MI, USA. Email: prbchiefstaff@med.wayne.eduSearch for more papers by this authorRoberto Romero, Corresponding Author Roberto Romero prbchiefstaff@med.wayne.edu Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, MI, USA Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, USA Correspondence Nardhy Gomez-Lopez, Perinatology Research Branch, NICHD/NIH/DHHS, Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. Email: ngomezlo@med.wayne.edu and Roberto Romero, Perinatology Research Branch, NICHD/NIH/DHHS, Hutzel Women's Hospital, Detroit, MI, USA. Email: prbchiefstaff@med.wayne.eduSearch for more papers by this authorYaozhu Leng, Yaozhu Leng Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USASearch for more papers by this authorValeria Garcia-Flores, Valeria Garcia-Flores Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USASearch for more papers by this authorYi Xu, Yi Xu Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USASearch for more papers by this authorDerek Miller, Derek Miller Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI, USASearch for more papers by this authorSonia S. Hassan, Sonia S. Hassan Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USASearch for more papers by this author Nardhy Gomez-Lopez, Corresponding Author Nardhy Gomez-Lopez ngomezlo@med.wayne.edu Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI, USA Correspondence Nardhy Gomez-Lopez, Perinatology Research Branch, NICHD/NIH/DHHS, Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. Email: ngomezlo@med.wayne.edu and Roberto Romero, Perinatology Research Branch, NICHD/NIH/DHHS, Hutzel Women's Hospital, Detroit, MI, USA. Email: prbchiefstaff@med.wayne.eduSearch for more papers by this authorRoberto Romero, Corresponding Author Roberto Romero prbchiefstaff@med.wayne.edu Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, MI, USA Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, USA Correspondence Nardhy Gomez-Lopez, Perinatology Research Branch, NICHD/NIH/DHHS, Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. Email: ngomezlo@med.wayne.edu and Roberto Romero, Perinatology Research Branch, NICHD/NIH/DHHS, Hutzel Women's Hospital, Detroit, MI, USA. Email: prbchiefstaff@med.wayne.eduSearch for more papers by this authorYaozhu Leng, Yaozhu Leng Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USASearch for more papers by this authorValeria Garcia-Flores, Valeria Garcia-Flores Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USASearch for more papers by this authorYi Xu, Yi Xu Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USASearch for more papers by this authorDerek Miller, Derek Miller Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI, USASearch for more papers by this authorSonia S. Hassan, Sonia S. Hassan Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH/DHHS, Bethesda, MD, and Detroit, MI, USA Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USASearch for more papers by this author First published: 03 January 2017 https://doi.org/10.1111/aji.12617Citations: 8Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Problem Neutrophil extracellular traps (NETs) were recently described as a mechanism for microbial killing in the amniotic cavity of women with intra-amniotic infection. Such a clinical condition can result in acute chorioamnionitis, a placental lesion characterized by the infiltration of maternal neutrophils in the chorioamniotic membranes. Herein, we investigated whether these infiltrating neutrophils form NETs in the chorioamniotic membranes from women who underwent spontaneous term or preterm labor with acute chorioamnionitis. Method of study Chorioamniotic membrane samples were collected from women who underwent spontaneous term or preterm labor with acute chorioamnionitis (n=10 each). Controls included chorioamniotic membrane samples from women who delivered at term or preterm with or without labor in the absence of acute chorioamnionitis (n=10 each). NETs were visualized and semiquantified in the chorioamniotic membranes by using antibodies against neutrophil elastase and histone H3 in combination with DAPI staining. Results Neutrophil extracellular traps were abundant in the chorioamniotic membranes from women who underwent spontaneous term or preterm labor with acute chorioamnionitis. NETs were rarely found, or not visualized at all, in the chorioamniotic membranes from women who delivered at term or preterm with or without labor in the absence of acute chorioamnionitis. Conclusion Neutrophil extracellular traps are abundant in the chorioamniotic membranes from women who underwent spontaneous term or preterm labor with acute chorioamnionitis. These findings suggest that chorioamniotic neutrophils can form NETs as a mechanism of host defense against infection or danger signals. Citing Literature Supporting Information Filename Description aji12617-sup-0001-Video1.wmvvideo/wmv, 2.9 MB aji12617-sup-0002-Video2.wmvvideo/wmv, 1.6 MB Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume77, Issue3March 2017e12617 RelatedInformation
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