Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics

2016; Nature Portfolio; Volume: 6; Issue: 1 Linguagem: Inglês

10.1038/srep38915

2045-2322

Luciana Principal Antunes, Layla Farage Martins, Roberta Verciano Pereira, Andrew Maltez Thomas, Deibs Barbosa, Leandro Nascimento Lemos, Gianluca Major Machado Silva, Livia Maria Silva Moura, George Willian Condomitti Epamino, Luciano Antônio Digiampietri, Karen Cristina Lombardi, Patrícia Locosque Ramos, Ronaldo Quaggio, Júlio Cézar Franco de Oliveira, Renata C. Pascon, João Batista da Cruz, Aline Maria da Silva, João Carlos Setúbal,

Studies on Chitinases and Chitosanases

Abstract Composting is a promising source of new organisms and thermostable enzymes that may be helpful in environmental management and industrial processes. Here we present results of metagenomic- and metatranscriptomic-based analyses of a large composting operation in the São Paulo Zoo Park. This composting exhibits a sustained thermophilic profile (50 °C to 75 °C), which seems to preclude fungal activity. The main novelty of our study is the combination of time-series sampling with shotgun DNA, 16S rRNA gene amplicon, and metatranscriptome high-throughput sequencing, enabling an unprecedented detailed view of microbial community structure, dynamics, and function in this ecosystem. The time-series data showed that the turning procedure has a strong impact on the compost microbiota, restoring to a certain extent the population profile seen at the beginning of the process; and that lignocellulosic biomass deconstruction occurs synergistically and sequentially, with hemicellulose being degraded preferentially to cellulose and lignin. Moreover, our sequencing data allowed near-complete genome reconstruction of five bacterial species previously found in biomass-degrading environments and of a novel biodegrading bacterial species, likely a new genus in the order Bacillales. The data and analyses provided are a rich source for additional investigations of thermophilic composting microbiology.