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First Report of Bacterial Stem Rot of Tomatoes Caused by Pectobacterium carotovorum subsp. brasiliense in Colombia

2016; American Phytopathological Society; Volume: 101; Issue: 5 Linguagem: Inglês

10.1094/pdis-08-16-1184-pdn

1943-7692

Alejandro Jaramillo, C. A. Huertas, E. Gómez,

Irrigation Practices and Water Management

HomePlant DiseaseVol. 101, No. 5First Report of Bacterial Stem Rot of Tomatoes Caused by Pectobacterium carotovorum subsp. brasiliense in Colombia PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Bacterial Stem Rot of Tomatoes Caused by Pectobacterium carotovorum subsp. brasiliense in ColombiaA. Jaramillo, C. A. Huertas, and E. D. GómezA. JaramilloSearch for more papers by this author, C. A. HuertasSearch for more papers by this author, and E. D. GómezSearch for more papers by this authorAffiliationsAuthors and Affiliations A. Jaramillo C. A. Huertas E. D. Gómez , Universidad Nacional de Colombia Sede Palmira, Facultad de Ciencias Agropecuarias, Palmira, Colombia. Published Online:14 Feb 2017https://doi.org/10.1094/PDIS-08-16-1184-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat During 2013 and 2014, farms in the municipality of La Cumbre, Valle del Cauca, that produce the ‘Calima’ hybrid tomato, Solanum lycopersicum L., were visited. Tomato plants with soft, aqueous lesions and brown lesions on the lower stem were observed. The vascular bundles were necrotic and in various states of decay, with some plants presenting cracking symptoms along the stem. At advanced stages of the disease, presence of exudates from the lesions was observed. Due to the destruction of medular tissue, the stems appeared hollow and brittle, resulting in symptoms of chlorosis and slight loss of turgidity in leaves. The incidence of the disease ranged from 5 to 80%. Isolation of the possible causal agents of this disease was done following the method described by Castaño and Mendoza (1997) and Botero et al. (2013). Seventeen bacterial isolates were obtained and were thereafter used in pathogenicity tests on 35-day-old tomato plants of the ‘Calima’ hybrid. Inoculation was performed by puncturing the stem with a bacterial suspension in sterile distilled water (108 CFU/ml) using a sterile syringe. All plants were kept in a moist chamber for 48 h. After 3 days, the plants that were inoculated with isolate LCB07 showed stems with aqueous brown lesions, softening and hollowing, and presence of exudation at the site of inoculation. Control plants, which were inoculated with sterile distilled water, showed no symptoms. When the pathogen was reisolated and identified using morphological, biochemical, and molecular methods, results confirmed the originally isolated pathogen. Molecular identification of the pathogenic isolate was performed by amplifying the 16S region of rRNA using universal primers (27f/Llr), and reconfirmed by amplification of 16S-23S intergenic space region of rRNA using primers (1491f/Llr) (Fessehaie et al. 2002). Alignment of study and reference sequences deposited in the NCBI GenBank database showed that the isolate LCB07 has 98% similarity with Pectobacterium carotovorum subsp. brasiliense. Unique sequences in this study were submitted to GenBank under accession numbers KX669672.1 and KX673747.1. This is the first report of P. carotovorum subsp. brasiliense causing bacterial stem rot of tomatoes in Colombia.References:Botero, M., et al. 2013. Manual práctico de bacteriología vegetal. Universidad de Caldas, Manizales, Colombia. Google ScholarCastaño, J., and Mendoza, L. 1997. Manual para el diagnóstico de hongos, bacterias, virus y nemátodos fitopatógenos. Universidad de Caldas, Manizales, Colombia. Google ScholarFessehaie, A., et al. 2002. Can. J. Microbiol. 48:387. https://doi.org/10.1139/w02-026 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 5 May 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 14 Apr 2017Published: 14 Feb 2017First Look: 19 Dec 2016Accepted: 10 Dec 2016 Page: 830 Information© 2017 The American Phytopathological SocietyCited byDickeya solani, Pectobacterium atrosepticum and Pseudomonas asplenii : causal agents of bacterial soft rot in cyclamen plants ( Cyclamen persicum Mill.) in Colombia3 March 2022 | Canadian Journal of Plant Pathology, Vol. 44, No. 4Pectobacterium brasiliense (soft rot and blackleg of ornamentals and potato)CABI Compendium, Vol. CABI CompendiumPectobacterium carotovorumCABI Compendium, Vol. CABI CompendiumPectobacterium brasiliense: Genomics, Host Range and Disease Management5 January 2021 | Microorganisms, Vol. 9, No. 1Diseases Caused by Pectobacterium and Dickeya Species Around the World5 January 2021Вacterial rot of tomatoes when grown in a protected ground11 November 2020 | Biological Systems: Theory and Innovation, Vol. 11, No. 3Development of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of Pseudomonas syringae pv. tomato in planta13 January 2020 | European Journal of Plant Pathology, Vol. 156, No. 3Pectobacteriumcarotovorum subsp. brasiliense Causes Soft Rot and Death of Neobuxbaumia tetetzo in Zapotitlan Salinas Valley, Puebla, MexicoDimas Mejía-Sánchez, Sergio Aranda-Ocampo, Cristian Nava-Díaz, Daniel Teliz-Ortiz, Manuel Livera-Muñoz, Rodolfo De La Torre-Almaráz, and Samuel Ramírez-Alarcón9 January 2019 | Plant Disease, Vol. 103, No. 3Pectobacterium carotovorum subsp. brasiliense and Pectobacterium carotovorum subsp. carotovorum as causal agents of potato soft rot in Algeria21 February 2018 | European Journal of Plant Pathology, Vol. 151, No. 4First Report of Bell Pepper Soft-Rot Caused by Pectobacterium carotovorum subsp. brasiliense in VenezuelaA. 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