Produção Nacional
Revisado por pares
Vaccinia virus in feces of wild rodents from São Paulo State, Brazil
2016; Elsevier BV; Volume: 53; Linguagem: Inglês
10.1016/j.ijid.2016.11.318
ISSN1878-3511
AutoresJane Megid, Marina Gea Peres, Tatiana Fernanda Bachiega, Camila Michele Appolinário, Acácia Ferreira Vicente, Bruna Letícia Devidé Ribeiro, Clóvis Rinaldo Fonseca, Jônatas Santos Abrahão, Fernando Ferreira,
Tópico(s)Herpesvirus Infections and Treatments
ResumoPurpose: The origin of Vaccinia virus (VACV) outbreaks in Brazil remains unknown, but since the isolation of VACV in Mus musculus mice during a zoonotic outbreak that peridomestic rodents have been suggested to be a link between cows and wild animals. Considering that experimentally infected mice eliminate viral particles in their feces, we investigated the presence of VACV in the feces of wild rodents captured in the forest areas surrounding milking farms in the central west region of São Paulo State, Brazil. Methods & Materials: Samples were collected in 47 farms randomly selected, including 10 in Torre de Pedra (23 14'58.76"S48 11'39.49"W) in which outbreaks were registered in 2007 and 2010, 15 in Bofete (23 05'54.51"S48 11'26.61"W) and 22 in Anhembi (23 05'54.51"S48 11'26.61"W), both without reports of outbreaks. Wild rodents were captured from May to September of 2011 in Pitfall and Sherman traps. The rodents were anesthetized in plastic autoclavable bags containing gauze soaked in ethyl ether. Blood samples, organs, and feces were collected. Viral DNA was extracted from feces using the RTP® DNA/RNA Virus Mini Kit (Stratec Molecular, Berlin, Germany). A nested PCR was used for amplification of the vaccinia growth factor (vgf) gene, and positive samples were submitted to gene sequencing. Results: Fecal samples were collected from 115 animals, of the following species captured: Akodon montensis, Calomys tener, Juliomys pictipes, Necromys lasiurus, Nectomys squamipes, Oligoryzomys flavescens, Oligoryzomys nigripes, and Sooretamys angouya. Six (5,2%) were positive including 14% of Sooretamys anoguya, 8% of Oligoryzomys flavescens, and 4% of Oligoryzomys nigripes., however, only one of the positive samples was sequenced because the low DNA concentration of the other five were not sufficient for sequencing. The sequencing and phylogenetic tree revealed that our strain clustered with the Brazilian VACVs Conclusion: For the first time, we reports detection of VACV DNA in feces of naturally infected Oligoryzomys flavescens, Oligoryzomys nigripes and Sooretamys angouya. Indeed, the, transmission of VACV via feces of experimentally infected mice to cows has not yet been described, and no cases of rodent-to-cow or rodent-to-human VACV transmission have been reported during the Brazilian outbreaks. Additional studies are needed to further elucidate this epidemiologic situation
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