First Report of the Detection of Bean yellow mosaic virus (BYMV) on Tropaeolum majus ; Hippeastrum spp., and Liatris spp. in South Africa
2016; American Phytopathological Society; Volume: 101; Issue: 5 Linguagem: Inglês
10.1094/pdis-10-16-1446-pdn
ISSN1943-7692
AutoresA. Schulze, Ronel Roberts, Gerhard Pietersen,
Tópico(s)Legume Nitrogen Fixing Symbiosis
ResumoHomePlant DiseaseVol. 101, No. 5First Report of the Detection of Bean yellow mosaic virus (BYMV) on Tropaeolum majus; Hippeastrum spp., and Liatris spp. in South Africa PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of the Detection of Bean yellow mosaic virus (BYMV) on Tropaeolum majus; Hippeastrum spp., and Liatris spp. in South AfricaA. Schulze, R. Roberts, and G. PietersenA. Schulze, R. Roberts, and G. PietersenAffiliationsAuthors and Affiliations A. Schulze , FABI, Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, 0002, South Africa R. Roberts G. Pietersen , FABI, Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, 0002, South Africa, and Agricultural Research Council-Plant Protection Research Institute, Pretoria, South Africa. Published Online:14 Feb 2017https://doi.org/10.1094/PDIS-10-16-1446-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat The potyvirus, Bean yellow mosaic virus (BYMV) is an economically important plant virus which infects many leguminous crops (family Fabaceae) as well as members of the Liliaceae. BYMV has been detected in South Africa on Freesia spp., Gladiolus hortulanus, Lathyrus odoratus, Lupinus albus, Viola odoratus (Gorter 1977), and Pisum sativum (Jooste et al. 2001), but few further studies have been conducted on this virus locally. During the current study, RT-PCR capable of generic detection of potyviruses (Zheng et al. 2010) was utilized to detect these viruses from plant samples submitted by growers and previously shown to contain potyvirus-like flexuous rod-shaped particles by electron microscopy. Plant material of these were deposited in a local virus repository (National Plant Virus and Antiserum Collection, Agriculture Research Council - Plant Protection Research Institute, Pretoria). The RT-PCR is directed at a 350-bp region of the nuclear inclusion body gene and the primer pair were the published NIb2F and NIb3R pair (5′-GTITGYGTIGAYGAYTTYAAYAA-3′; 5′-TCIACIACIGTIGAIGGYTGNCC-3′). All samples yielding amplicons of the expected size from the RT-PCR reaction were subjected to Sanger sequencing. Five samples (accessions 91/0266, 92/0259, 94/1815, 95/0987, 95/0971) had nucleotide sequences indicative of BYMV infection when aligned to the cognate region of 48 potyvirus sequences previously deposited in GenBank and occurred in the same clade as BYMV in maximum likelihood derived dendrograms derived from these alignments. The sources had, respectively, 90, 94, 94, 97, and 99% nucleotide identity with various BYMV strains. The presence of BYMV in these samples was confirmed by F(ab')2 ELISA using antiserum specific to BYMV-Scott (Barnett et al. 1984) with BYMV-Scott itself used as positive control (average OD405nm = 2.33). All five samples yielded absorbance values (average OD405nm between 1.044 and 1.893), which were higher than at least two standard deviations above the mean of the healthy control (OD405nm > 0.517), thereby confirming the presence of BYMV. This identification is therefore based on the initial presence of potyvirus-like particles, amplification with generic potyvirus PCR primers, nucleotide sequence of the amplicons obtained, and also by a positive reaction in ELISA. This confirms the previous finding in South Africa of BYMV from L. albus (sample 94/1815 collected April 1994 from Pietermaritzburg; GenBank accession KX907126), infection of BYMV on Freesia spp. (sample 95/0971; collected July 1995 from the Quarantine Station, Marikana, GenBank accession KX907125), but also reports the infection of BYMV on Liatrus spp. (sample 95/0987 collected July 1995 from Pretoria; GenBank accession KX757143) and Hippeastrum spp. (sample 91/0259 collected July 1991 from Brits; GenBank accession KX907124), and for the first time in this country and internationally on Tropaeolum majus (sample 91/0266 collected July 1991 from Pietermaritzburg; GenBank accession KX757144).References:Barnett, O. W., et al. 1984. VI International Symposium on Virus Diseases of Ornamental Plants 164. Google ScholarGorter, G. J. M. A. 1977. Index of Plant Pathogens and the Diseases they Cause in Cultivated Plants in South Africa. Science Bulletin 392, Department of Agricultural Technical Services, South Africa. Google ScholarJooste, A. E. C., et al. 2001. Afr. Plant Prot. 7:59. Google ScholarZheng, L., et al. 2010. Plant Pathol. 59:211. https://doi.org/10.1111/j.1365-3059.2009.02201.x Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 5 May 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 14 Apr 2017Published: 14 Feb 2017First Look: 27 Dec 2016Accepted: 15 Dec 2016 Page: 846 Information© 2017 The American Phytopathological SocietyCited byUnraveling the Tropaeolum majus L. 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