First Report of Pedilanthus leaf curl virus , Tobacco leaf curl betasatellite, and Guar leaf curl alphasatellite Infecting Radish ( Raphanus sativus ) in Pakistan
2017; American Phytopathological Society; Volume: 101; Issue: 5 Linguagem: Inglês
10.1094/pdis-09-16-1265-pdn
ISSN1943-7692
AutoresH. Ismail, Ishtiaq Hassan, Muhammad Zubair, Muhammad Zuhaib Khan, Saad Serfraz, N. Jamil, Shahid Mansoor, Shaheen Asad, Imran Amin,
Tópico(s)Animal Disease Management and Epidemiology
ResumoHomePlant DiseaseVol. 101, No. 5First Report of Pedilanthus leaf curl virus, Tobacco leaf curl betasatellite, and Guar leaf curl alphasatellite Infecting Radish (Raphanus sativus) in Pakistan PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pedilanthus leaf curl virus, Tobacco leaf curl betasatellite, and Guar leaf curl alphasatellite Infecting Radish (Raphanus sativus) in PakistanH. Ismail, I. Hassan, M. Zubair, M. Z. Khan, S. Serfraz, N. Jamil, S. Mansoor, S. Asad, and I. AminH. IsmailSearch for more papers by this author, I. HassanSearch for more papers by this author, M. ZubairSearch for more papers by this author, M. Z. KhanSearch for more papers by this author, S. SerfrazSearch for more papers by this author, N. JamilSearch for more papers by this author, S. MansoorSearch for more papers by this author, S. AsadSearch for more papers by this author, and I. AminSearch for more papers by this authorAffiliationsAuthors and Affiliations H. Ismail I. Hassan M. Zubair M. Z. Khan S. Serfraz N. Jamil S. Mansoor S. Asad I. Amin , National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan. Published Online:23 Feb 2017https://doi.org/10.1094/PDIS-09-16-1265-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Begomoviruses are whitefly (Bemisia tabaci)-transmitted single-stranded DNA viruses of the family Geminiviridae that cause extensive crop losses across the warmer parts of the world. During a survey in October 2015 to monitor begomovirus infection in radish (Raphanus sativus), plants were noted with symptoms typical of begomovirus infection, consisting of upward leaf curling, stunted growth, and vein yellowing in the Chiniot region of Punjab province. Symptoms were observed in three fields and infection ranged from 10 to 20%. Leaf samples were collected from seven symptomatic and five non-symptomatic radish plants and total genomic DNA was extracted using the CTAB method (Doyle and Doyle 1990). PCR with a primer pair designed to amplify all begomoviruses (BegomoF/BegomoR; Shahid et al. 2007) resulted in amplification of product of the expected size (∼2.8 kb) from one out of the seven symptomatic samples while no amplification was observed in healthy tissues. The amplified product was cloned in pTZ57R/T (Thermo Scientific, U.S.A.), sequenced and analyzed through nBLAST (www.ncbi.nlm.nih.gov/blast). The results showed the sequence of the clone to have the highest nucleotide sequence identity (94%) with an isolate of Pedilanthus leaf curl virus (PeLCV) from spinach originating from Pakistan (accession no. HF568781). The sequence is available in the nucleotide sequence database under the accession number LT600729. To further confirm the presence of a begomovirus, total DNA from five symptomatic (including the plant from which the clone was obtained) and one non-symptomatic plant were resolved on agarose gel, transferred to nylon membrane, and hybridized with DIG-labeled PeLCV probe produced by PCR with primer pair CLCV1/CLCV2 (Hussain et al. 2003). Hybridization was detected for two symptomatic samples (including the one positive by PCR) but not for the sample lacking symptoms. The radish samples were also assessed for the presence of alpha- and betasatellites. PCR with universal primer pair for amplification of alphasatellites (DNA101/DNA102; Bull et al. 2003) yielded a product of the expected ∼1.4 kb from one symptomatic sample (from which the virus was cloned). The fragment was cloned and sequenced. The sequence was 1,358 bp in length and an nBLAST analysis showed 98% sequence identity with an isolate of Guar leaf curl alphasatellite (GuLCA: HGA17075). Similarly, PCR with a primer pair designed to amplify all betasatellites (beta01/beta02; Briddon et al. 2002) resulted in the expected ∼1.4 kb product for one sample (from which the alphasatellite and virus were cloned). This sequence was 1,363 bp in length and showed 97% identity with an isolate of Tobacco leaf curl Pakistan betasatellite (TbLCB: AM922485) upon nBLAST. The sequences of alpha- and betasatellites are available in the nucleotide sequence databases under accession numbers LT600724 and LT600728, respectively. Southern blot analyses were conducted using the cloned satellites as probes, which showed the presence of both satellites in the two samples in which PeLCV was detected. To the best of our knowledge, this is the first report of PeLCV with TbLCB and GuLCA infecting radish in Pakistan.References:Briddon, R. W., et al. 2002. Mol. Biotechnol. 20:315. https://doi.org/10.1385/MB:20:3:315 Crossref, ISI, Google ScholarBull, S. et al. 2003. Mol. Biotechnol. 23:83. Google ScholarDoyle, J. J., and Doyle, J. L. 1990. Focus 12:13. Google ScholarHussain, M. et al. 2003. Plant Pathol. 52:809. Google ScholarShahid, M. et al. 2007. Arch Virol. 152:2131. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 5 May 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 14 Apr 2017Published: 23 Feb 2017First Look: 9 Jan 2017Accepted: 23 Dec 2016 Pages: 845-845 Information© 2017 The American Phytopathological SocietyCited byAnalysis of the effects of the mutation of selected genes of pedilanthus leaf curl virus on infectivity, symptoms and the maintenance of tobacco leaf curl betasatellite25 May 2022 | Canadian Journal of Plant Pathology, Vol. 44, No. 5Tobacco leaf curl betasatelliteCABI Compendium, Vol. CABI CompendiumPedilanthus leaf curl virusCABI Compendium, Vol. CABI CompendiumAssociation of an isolate of papaya leaf curl virus and papaya leaf curl betasatellite with leaf curl disease of radish in India26 January 2021 | Journal of Plant Pathology, Vol. 103, No. 2Interaction of eukaryotic proliferating cell nuclear antigen (PCNA) with the replication-associated protein (Rep) of cotton leaf curl Multan virus and pedilanthus leaf curl virus2 January 2021 | 3 Biotech, Vol. 11, No. 1Codiaeum variegatum in Pakistan harbours pedilanthus leaf curl virus and papaya leaf curl virus as well as a newly identified betasatellite23 May 2020 | Archives of Virology, Vol. 165, No. 8Identification of two further agriculturally important begomoviruses and their associated satellites infecting the weed Digera arvensis in Pakistan14 June 2019 | European Journal of Plant Pathology, Vol. 155, No. 2Raphanus sativus (Radish)6 June 2020Characterization, phylogeny and recombination analysis of Pedilanthus leaf curl virus-Petunia isolate and its associated betasatellite31 August 2018 | Virology Journal, Vol. 15, No. 1Identification of pedilanthus leaf curl virus and associated betasatellite infecting turnip in Pakistan24 May 2018 | Journal of Plant Pathology, Vol. 100, No. 2
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