Produção Nacional
Revisado por pares
Epiisopiloturine hydrochloride, an imidazole alkaloid isolated from Pilocarpus microphyllus leaves, protects against naproxen-induced gastrointestinal damage in rats
2017; Elsevier BV; Volume: 87; Linguagem: Inglês
10.1016/j.biopha.2016.12.101
ISSN1950-6007
AutoresLucas Antônio Duarte Nicolau, Nathalia Santos Carvalho, Dvison M. Pacífico, Larisse T. Lucetti, Karoline S. Aragão, Leiz Maria Costa Véras, Marcellus Henrique Loiola Ponte de Souza, J. R. Leite, Jand Venes Rolim Medeiros,
Tópico(s)Helicobacter pylori-related gastroenterology studies
ResumoThis study aimed to investigate the protective effect of epiisopiloturine hydrochloride (EPI), an imidazole alkaloid, on NAP-induced gastrointestinal damage in rats. Initially, rats were pretreated with 0.5% carboxymethylcellulose (vehicle) or EPI (3, 10 and 30 mg/kg, p.o. or i.p., groups 3–5, respectively) twice daily, for 2 days. After 1 h, NAP (80 mg/kg, p.o.) was given. The control group received only vehicle (group 1) or vehicle + naproxen (group 2). Rats were euthanized on 2nd day, 4 h after NAP treatment. Stomachs lesions were measured. Samples were collected for histological evaluation and glutathione (GSH), malonyldialdehyde (MDA), myeloperoxidase (MPO), and cytokines levels. Moreover, gastric mucosal blood flow (GMBF) was evaluated. EPI pretreatment prevented NAP-induced macro and microscopic gastric damage with a maximal effect at 10 mg/kg. Histological analysis revealed that EPI decreased scores of damage caused by NAP. EPI reduced MPO (3.4 ± 0.3 U/mg of gastric tissue) and inhibited changes in MDA (70.4 ± 8.3 mg/g of gastric tissue) and GSH (246.2 ± 26.4 mg/g of gastric tissue). NAP increased TNF-α levels, and this effect was reduced by EPI pretreatment. Furthermore, EPI increased GMBF by 15% compared with the control group. Our data show that EPI protects against NAP-induced gastric and intestinal damage by reducing pro-inflammatory cytokines, reducing oxidative stress, and increasing GMBF.
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