Portal de Periódicos da CAPES

In vitro dentin barrier cytotoxicity testing of some dental restorative materials

2017; Elsevier BV; Volume: 58; Linguagem: Inglês

10.1016/j.jdent.2017.01.003

1879-176X

Ruodan Jiang, Hong Lin, Gang Zheng, Xiucai Zhang, Qianqian Du, Ming Yang,

Dental Implant Techniques and Outcomes

To investigate the cytotoxicity of four dental restorative materials in three-dimensional (3D) L929 cell cultures using a dentin barrier test. The cytotoxicities of light-cured glass ionomer cement (Vitrebond), total-etching adhesive (GLUMA Bond5), and two self-etching adhesives (GLUMA Self Etch and Single Bond Universal) were evaluated. The permeabilities of human dentin disks with thicknesses of 300, 500, and 1000 μm were standardized using a hydraulic device. Test materials and controls were applied to the occlusal side of human dentin disks. The 3D-cell scaffolds were placed beneath the dentin disks. After a 24-h contact with the dentin barrier test device, cell viabilities were measured by performing MTT assays. Statistical analysis was performed using the Mann–Whitney U test. The mean (SD) permeabilities of the 300-μm, 500-μm, and 1000-μm dentin disks were 0.626 (0.214), 0.219 (0.0387) and 0.089 (0.028) μl min−1 cm−2 cm H2O−1. Vitrebond was severely cytotoxic, reducing the cell viability to 10% (300-μm disk), 17% (500 μm), and 18% (1000 μm). GLUMA Bond5 reduced the cell viability to 40% (300 μm), 83% (500 μm), and 86% (1000 μm), showing moderate cytotoxicity (300-μm) and non-cytotoxicity (500-μm and 1000-μm). Single Bond Universal and GLUMA Self Etch did not significantly reduce cell viability, regardless of the dentin thicknesses, which characterized them as non-cytotoxic. Cytotoxicity varied with the materials tested and the thicknesses of the dentin disks. The tested cytotoxicity of materials applied on 300-, 500-, and 1000-μm dentin disks indicates that the clinical use of the test materials (excepting self-etching adhesives) in deep cavities poses a potential risk of damage to the pulp tissues to an extent, depending on the thickness of the remaining dentin.