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Application of Z-sinapinic matrix in peptide MALDI-MS analysis

2017; Wiley; Volume: 52; Issue: 3 Linguagem: Inglês

10.1002/jms.3908

1096-9888

María L. Salum, Silvana L. Giudicessi, Tobías Schmidt De León, Silvia A. Camperi, Rosa Erra‐Balsells,

Advanced Proteomics Techniques and Applications

Journal of Mass SpectrometryVolume 52, Issue 3 p. 182-186 Application note Application of Z-sinapinic matrix in peptide MALDI-MS analysis M. L. Salum, M. L. Salum CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428 Buenos Aires, Argentina These authors contributed equally to this work.Search for more papers by this authorS. L. Giudicessi, S. L. Giudicessi Consejo Nacional de lnvestigaciones Cientificas y Técnicas, Instituto de Nanobiotecnología (NANOBIOTEC), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina These authors contributed equally to this work.Search for more papers by this authorT. Schmidt De León, T. Schmidt De León CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428 Buenos Aires, ArgentinaSearch for more papers by this authorS. A. Camperi, S. A. Camperi Consejo Nacional de lnvestigaciones Cientificas y Técnicas, Instituto de Nanobiotecnología (NANOBIOTEC), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, ArgentinaSearch for more papers by this authorR. Erra-Balsells, Corresponding Author R. Erra-Balsells erra@qo.fcen.uba.ar orcid.org/0000-0003-0169-0173 CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428 Buenos Aires, ArgentinaCorrespondence to: Rosa Erra-Balsells, CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428 Buenos Aires, Argentina. E-mail: erra@qo.fcen.uba.arSearch for more papers by this author M. L. Salum, M. L. Salum CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428 Buenos Aires, Argentina These authors contributed equally to this work.Search for more papers by this authorS. L. Giudicessi, S. L. Giudicessi Consejo Nacional de lnvestigaciones Cientificas y Técnicas, Instituto de Nanobiotecnología (NANOBIOTEC), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina These authors contributed equally to this work.Search for more papers by this authorT. Schmidt De León, T. Schmidt De León CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428 Buenos Aires, ArgentinaSearch for more papers by this authorS. A. Camperi, S. A. Camperi Consejo Nacional de lnvestigaciones Cientificas y Técnicas, Instituto de Nanobiotecnología (NANOBIOTEC), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, ArgentinaSearch for more papers by this authorR. Erra-Balsells, Corresponding Author R. Erra-Balsells erra@qo.fcen.uba.ar orcid.org/0000-0003-0169-0173 CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428 Buenos Aires, ArgentinaCorrespondence to: Rosa Erra-Balsells, CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428 Buenos Aires, Argentina. E-mail: erra@qo.fcen.uba.arSearch for more papers by this author First published: 14 January 2017 https://doi.org/10.1002/jms.3908Citations: 9 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here, Z-SA as matrix for peptides is studied and compared with E-SA and α-cyano-4-hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright © 2017 John Wiley & Sons, Ltd. Citing Literature Supporting Information Filename Description jms3908-sup-0001-SI.docxWord 2007 document , 199 KB Figure S1. Supporting info item jms3908-sup-0002-SI.docxWord 2007 document , 3 MB Figure S2. Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume52, Issue3March 2017Pages 182-186 RelatedInformation