Cholesterol and ergosterol affect the activity of Staphylococcus aureus antibiotic efflux pumps
2017; Elsevier BV; Volume: 104; Linguagem: Inglês
10.1016/j.micpath.2017.01.019
ISSN1096-1208
AutoresSaulo Relison Tintino, Cícera Datiane de Morais Oliveira‐Tintino, Fábia F. Campina, Maria do Socorro Costa, Rafael Pereira da Cruz, Raimundo Luíz Silva Pereira, Jacqueline Cosmo Andrade, Erlânio Oliveira de Sousa, José P. Siqueira-Júnior, Henrique Douglas Melo Coutinho, Tereza Cristina Leal‐Balbino, Valdir de Queiroz Balbino,
Tópico(s)Antibiotic Resistance in Bacteria
ResumoThe aim of this study is to evaluate the effect of ergosterol on steroids and cholesterol efflux pumps in multidrug resistant strains of S. aureus. Were used RN4220 harboring plasmid pUL5054, which carries the gene encoding the MsrA macrolide efflux protein; and IS-58, which possesses the TetK tetracycline efflux protein; 1199B resists hydrophilic fluoroquinolones via a NorA-mediated mechanism and wild strain 1199B. The Minimal Inhibitory Concentration (MIC) was determined and the evaluation of possible inhibition of efflux pumps by reduction of MIC. Some of the detrimental effects on bacterial cells can be attributed to the detergent properties of cholesterol and ergosterol on account of their amphipathic structure. Besides the cholesterol did not affect directly the pump structure, a synergism was observed, maybe due the interaction with the cell membrane and interference in the lipid bilayer.
Referência(s)