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Eine Glucose-Dehydrogenase für die Glucose-Bestimmung in Körperflüssigkeiten

1975; De Gruyter; Volume: 13; Issue: 3 Linguagem: Inglês

10.1515/cclm.1975.13.3.101

ISSN

1437-4331

Autores

D Banauch, W. Brümmer, W. Ebeling, Harald Metz, H. Rindfrey, Hermann Lang, K. Leybold, W. Rick,

Tópico(s)

Polyamine Metabolism and Applications

Resumo

The isolation of glucose dehydrogenase from Bacillus megaterium M 1286 is outlined. Data on the specificity of the enzyme towards carbohydrates are given. A specific method for glucose determination using this enzyme was developed. Methods and results of four variants of this glucose determination are presented: End point determination in the UV range, determination with formazan as reaction product, kinetic determination in the UV range, and continuous flow analysis in the UV range (AutoAnalyzer method).

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