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Rapid adaptation drives invasion of airway donor microbiota by Pseudomonas after lung transplantation

2017; Nature Portfolio; Volume: 7; Issue: 1 Linguagem: Inglês

10.1038/srep40309

2045-2322

Marie Beaume, Thilo Köhler, Gilbert Greub, Oriol Manuel, John‐David Aubert, Loïc Baerlocher, Laurent Farinelli, Angus Buckling, Christian van Delden, Rita Achermann, Patrizia Amico, Philippe Baumann, Guido Beldi, Christian Benden, Christoph Berger, Isabelle Binet, Pierre‐Yves Bochud, Elsa Boely, Heiner C. Bucher, Léo H. Bühler, Thierry Carell, Oriol Manuel, Yves Chalandon, Sabina De Geest, Olivier de Rougemont, Michael Dickenmann, Michel Duchosal, Thomas Fehr, Sylvie Ferrari‐Lacraz, Christian Garzoni, Paola Gasche Soccal, Emiliano Giostra, Déla Golshayan, Daniel Good, Karine Hadaya, Jörg Halter, Dominik Heim, Christoph Hess, Sven Hillinger, Hans H. Hirsch, Günther F.L. Hofbauer, Uyen Huynh‐Do, Franz Immer, Richard Klaghofer, Michael Koller, Bettina Laesser, Roger Lehmann, Christian Lovis, Hans‐Peter Marti, Pierre Yves Martin, Luca Martinolli, Pascal Meylan, Paul Mohaçsi, Isabelle Morard, Philippe Morel, Ulrike Mueller, Nicolas J. Mueller, Helen Mueller-McKenna, A Müller, Thomas Müller, Beat Müllhaupt, David Nadal, Manuel Pascual, Jakob Passweg, Chantal Piot Ziegler, Juliane Rick, Eddy Roosnek, Anne Rosselet, Silvia Rothlin, Frank Ruschitzka, Urs Schanz, Stefan Schaub, Christian Seiler, Susanne Stampf, Jürg Steiger, Guido Stirnimann, Christian Toso, Dimitri Tsinalis, Jean-Pierre Venetz, Jean Villard, Madeleine Wick, Markus J. Wilhelm, Patrick Yerly,

Bacterial biofilms and quorum sensing

Abstract In cystic fibrosis (CF) patients, chronic airway infection by Pseudomonas leads to progressive lung destruction ultimately requiring lung transplantation (LT). Following LT, CF-adapted Pseudomonas strains, potentially originating from the sinuses, may seed the allograft leading to infections and reduced allograft survival. We investigated whether CF-adapted Pseudomonas populations invade the donor microbiota and adapt to the non-CF allograft. We collected sequential Pseudomonas isolates and airway samples from a CF-lung transplant recipient during two years, and followed the dynamics of the microbiota and Pseudomonas populations. We show that Pseudomonas invaded the host microbiota within three days post-LT, in association with a reduction in richness and diversity. A dominant mucoid and hypermutator mutL lineage was replaced after 11 days by non-mucoid strains. Despite antibiotic therapy, Pseudomonas dominated the allograft microbiota until day 95. We observed positive selection of pre-LT variants and the appearance of novel mutations. Phenotypic adaptation resulted in increased biofilm formation and swimming motility capacities. Pseudomonas was replaced after 95 days by a microbiota dominated by Actinobacillus . In conclusion, mucoid Pseudomonas adapted to the CF-lung remained able to invade the allograft. Selection of both pre-existing non-mucoid subpopulations and of novel phenotypic traits suggests rapid adaptation of Pseudomonas to the non-CF allograft.