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First Report of Tobacco vein banding mosaic virus Infecting Sesame in China

2017; American Phytopathological Society; Volume: 101; Issue: 5 Linguagem: Inglês

10.1094/pdis-12-16-1707-pdn

1943-7692

H. Y. Wang, Huanran Gong, Tongyan Tian, Wei Tang, Tiansheng Zhu, Ming Zhao, Xiangdong Li,

Plant and Fungal Interactions Research

HomePlant DiseaseVol. 101, No. 5First Report of Tobacco vein banding mosaic virus Infecting Sesame in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Tobacco vein banding mosaic virus Infecting Sesame in ChinaH. Y. Wang, H. H. Gong, Z. Y. Yan, W. Tang, T. S. Zhu, M. Zhao, and X. D. LiH. Y. WangSearch for more papers by this author, H. H. GongSearch for more papers by this author, Z. Y. YanSearch for more papers by this author, W. TangSearch for more papers by this author, T. S. ZhuSearch for more papers by this author, M. ZhaoSearch for more papers by this author, and X. D. LiSearch for more papers by this authorAffiliationsAuthors and Affiliations H. Y. Wang , Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai’an, Shandong 271018, China, and Cotton Research Center, Shandong Academy of Agricultural Sciences, Ji’nan, Shandong 250100, China H. H. Gong , Cotton Research Center, Shandong Academy of Agricultural Sciences, Ji’nan, Shandong 250100, China Z. Y. Yan W. Tang , Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai’an, Shandong 271018, China T. S. Zhu , Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai’an, Shandong 271018, China, and College of Plant Science and Technology, Tarim University, Alar, Xinjiang 843300, China M. Zhao , Cotton Research Center, Shandong Academy of Agricultural Sciences, Ji’nan, Shandong 250100, China X. D. Li , Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai’an, Shandong 271018, China. Published Online:10 Mar 2017https://doi.org/10.1094/PDIS-12-16-1707-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Sesame (Sesamum indicum) is one of the major economic crops in the world and is mainly cultivated in Asia and Africa. In the summer of 2016, we conducted a series of field surveys in Zhangqiu, Shandong Province, China, to investigate the viruses infecting sesame. Sesame plants (cv. Zhongzhi No. 11) showing symptoms of mosaic, etiolation, shoestring, and puckering were found in several fields at incidences of 20 to 35%.Young leaves of five symptomatic plants were collected for total RNA extraction using Trizol reagent (Invitrogen, Carlsbad, CA). Equal amounts of total RNAs were pooled for small-RNA deep-sequencing (SRDS) analysis (Illumina HisEquation 2500) to detect potential viruses. SRDS on the pooled RNA resulted in 26,181,653 reads. Clear reads of 21 to 24 nucleotides (nt) were assembled with Trinity v2.06 and used to search homologous sequences in the NCBI nucleotide database using the BLAST tool. BLAST searches identified three potyviruses, Watermelon mosaic virus (WMV), Bean common mosaic virus (BCMV), and Tobacco vein banding mosaic virus (TVBMV). The percentages of the small RNA reads corresponding to WMV, BCMV, and TVBMV were 0.43, 13.48, and 1.23%, respectively. WMV and BCMV have already been reported to infect sesame in China. The contigs mapping to TVBMV were 9,240 nt, covering 96.5% of its complete genome. To further confirm the existence of TVBMV in sesame samples, the 3′-terminal region of TVBMV genome was amplified by RT-PCR using primers specific to potyviruses (Chen et al. 2001). A fragment of ∼1.6 kb was amplified from three of the 12 plants tested. The resultant consensus sequences of 1,624 nt (GenBank accession no. KY247146, isolate name TVBMV-ZhangQiu) included 624 nt encoding partial NIb, 816 nt encoding CP, and 184 nt belonging to the 3′-UTR. The 3′-terminal genomic sequence of TVBMV-ZhangQiu shared identities of 90.64 to 98.09% at the nucleotide level with other TVBMV isolates available in GenBank, while the CP-encoding sequence shared identities of 90.04 to 98.52% at the nucleotide level and 95.93 to 100.0% at the amino acid level with other TVBMV isolates. TVBMV-ZhangQiu formed a clade with isolate GSZN5 (GU904069) from Gansu, China, and was clustered to the CPMC group (Zhang et al. 2011) in the phylogenetic analysis. In Western blot analysis, all the three PCR-positive plants showed positive reaction with antiserum against TVBMV (Lan et al. 2007). TVBMV has been reported to infect tobacco, potato, Datura stramonium, wild eggplant (Solanum torvum), and tomato in China (Geng et al. 2014; Roggero et al. 2000; Zhang et al. 2011). To the best of our knowledge, this is the first report that TVBMV can naturally infect sesame in China. Our results show that TVBMV is a potential threat to sesame production and provide new clues for the sustainable control of sesame viral diseases.References:Chen, J., et al. 2001. Arch. Virol. 146:757. https://doi.org/10.1007/s007050170144 Crossref, ISI, Google ScholarGeng, C. et al. 2014. J. Plant Pathol. 96:S4.127 Google ScholarLan, Y. F., et al. 2007. Acta Phytopathol. Sin. 37:461. Google ScholarRoggero, P., et al. 2000. Plant Dis. 84:1152. https://doi.org/10.1094/PDIS.2000.84.10.1152D Link, Google ScholarZhang, C. L., et al. 2011. Virus Res. 158:188. https://doi.org/10.1016/j.virusres.2011.03.031 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 5 May 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 14 Apr 2017Published: 10 Mar 2017First Look: 17 Jan 2017Accepted: 10 Jan 2017 Pages: 850-850 Information© 2017 The American Phytopathological SocietyCited byTobacco vein banding mosaic virusCABI Compendium, Vol. CABI CompendiumWatermelon mosaic virus (watermelon mosaic)CABI Compendium, Vol. CABI CompendiumBean common mosaic virus (common mosaic of beans)CABI Compendium, Vol. CABI CompendiumFirst Report of Tobacco Vein Banding Mosaic Virus Naturally Infecting Black Nightshade (Solanum nigrum) in ChinaJ. A. Bi, H. Y. Zheng, Y. W. Lu, J. J. Peng, L. Lin, Z. Chen, B. A. Song, J. P. Chen, and F. Yan12 February 2020 | Plant Disease, Vol. 104, No. 4Pest categorisation of non‐EU viruses and viroids of potatoEFSA Journal, Vol. 18, No. 1Sesamum indicum (Sesame)6 June 2020