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BIBTEX RIS

Effects of chitosan-coated lipid-core nanocapsules on bovine sperm cells

2017; Elsevier BV; Volume: 40; Linguagem: Inglês

10.1016/j.tiv.2017.01.017

1879-3177

Arthur de Castro Jorge Silva, Mariana Härter Remirão, Caroline Gomes Lucas, William Borges Domingues, Tony Silveira, Júlia Damé Fonseca Paschoal, Denise Soledade Jornada, Carine Dahl Corcine, Antônio Sérgio Varela, Willian Andrade Prado, Vinícius Farias Campos, Fabiana K. Seixas, Sı́lvia Stanisçuaski Guterres, Adriana Raffin Pohlmann, Tiago Collares,

Nanoparticle-Based Drug Delivery

Toxicology studies have a pivotal role for selection of new nanosystems. As lipid-core nanocapsules (LNC) rise as a potential system not only for drug delivery but also for immunotherapy and gene therapy, the demand for models of toxic screening increases, and sperm arises as a promising model due to the easiness to evaluate its viability parameters. LNCs were coated with chitosan, chitosan-coated lipid-core nanocapsules (LNC-CS), in order to modify the nanocapsule surface. We evaluated the toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations (2.5%, 5%, 10%, 20%, 40% and 80%) (v/v) and periods of exposure (0h and 1h). CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects. The results corroborated with previous studies indicating that there is no toxicity from LNCs and LNC-CS below 40% (v/v) concentration.