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A Polymorphism in the <b><i>MTRR</i></b> Gene Is Associated with Early Childhood Caries and Underweight

2017; Karger Publishers; Volume: 51; Issue: 2 Linguagem: Inglês

10.1159/000451037

1421-976X

Lívia Azeredo Alves Antunes, Claudio Manoel Cabral Machado, Ana Carolina Kuntz Couto, Ludiana Barbosa Lopes, Fernanda Cunha Sena, Fernanda Volpe de Abreu, Renato Silva Fraga, Érika Calvano Küchler, Leonardo Santos Antunes,

Diet and metabolism studies

Polymorphisms in genes encoding the enzymes involved in the metabolism of homocysteine, such as methionine synthase (<i>MTR</i>) and methionine synthase reductase (<i>MTRR</i>), play an important function in the metabolism of folic acid and vitamin B₁₂. The present study aimed to evaluate the association of polymorphisms in genes <i>MTR </i>(rs1805087) and <i>MTRR</i> (rs1801394) with susceptibility of early childhood caries (ECC) and with body mass index alterations. A cross-sectional study was performed in 488 children aged from 2 to 6 years from 25 public day care centers in Rio de Janeiro, Brazil. Demographic data and oral health habits were obtained through a questionnaire. Anthropometric measurements and caries experience data were collected by 2 examiners (&#x03BA; = 0.80). Genotyping of the selected polymorphisms was carried out by TaqMan real-time PCR using genomic DNA extracted from buccal cells. Allele and genotype frequencies were compared between groups with and without disease. The <i>t</i> test, &#x03C7;² test, odds ratio, Pearson correlation tests, and logistic regression analysis were used (<i>p </i>≤ 0.05). The mean white spot lesion score was 1.18 (±2.57) in normal weight children and 2.50 (±3.87) in underweight children (<i>p</i> = 0.05). For <i>MTRR</i> polymorphisms, significant differences were observed for allele and genotype frequency distributions between caries-free and caries-affected children (<i>p</i> = 0.03 and 0.04 for allele and genotype frequencies, respectively) and in the genotype frequencies between normal weight and underweight children (<i>p</i> = 0.04). Our results suggest an association between underweight and ECC; in addition it is suggested that <i>MTRR</i> is a common genetic risk factor for ECC and underweight.