
Combination of natural antimicrobials and sodium dodecyl sulfate for disruption of biofilms formed by contaminant bacteria isolated from sugarcane mills
2017; Elsevier BV; Volume: 41; Linguagem: Inglês
10.1016/j.ifset.2017.01.007
ISSN1878-5522
AutoresRachel Tereza Rigotti, Jessica Audrey Feijó Corrêa, Natalia Janaina Lago Maia, Giovanna Cesaro, Edvaldo Antônio Ribeiro Rosa, Renata Ernlund Freitas de Macedo, Fernando Bittencourt Luciano,
Tópico(s)Advanced Chemical Sensor Technologies
ResumoBacterial contamination is found during ethanol production in the form of biofilms, which can decrease ethanol yield by 30%. Carvacrol, nisin, and hops extract, alone and in combination with SDS, were tested against planktonic and preformed biofilms of Lactobacillus fermentum, Lactobacillus plantarum, and/or Leuconostoc mesenteroides. MICs of carvacrol, nisin, hops extract and SDS were 250, 30, 5 and 37.5 mg/L, respectively, against all bacteria. Synergism between SDS and carvacrol (FICindex = 0.125), and SDS and nisin (FICindex = 0.25) was also found against all bacteria. Bacterial viability within biofilms and removal of biomass were indirectly measured using MTT and crystal violet methods, respectively. Metabolic activity of biofilms was inhibited up to 92% with nisin + SDS, while carvacrol + SDS could remove 82% of the biomass. Thus, combined use of these antimicrobials and SDS may provide an effective method for cleaning equipment of ethanol plants and prevent formation of biofilms.
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