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BIBTEX RIS

CasuL: A new lectin isolated from Calliandra surinamensis leaf pinnulae with cytotoxicity to cancer cells, antimicrobial activity and antibiofilm effect

2017; Elsevier BV; Volume: 98; Linguagem: Inglês

10.1016/j.ijbiomac.2017.02.019

ISSN

1879-0003

Autores

Thamara Figueiredo Procópio, Leydianne Leite de Siqueira Patriota, Maiara Celine de Moura, Pollyanna Michelle da Silva, Ana Patrícia Silva de Oliveira, Lidiane Vasconcelos do Nascimento Carvalho, Thâmarah de Albuquerque Lima, Tatiana Soares, Túlio Diego da Silva, Luana Cassandra Breitenbach Barroso Coelho, Maíra Galdino da Rocha Pitta, Moacyr Jesus Barreto de Melo Rêgo, Regina Célia Bressan Queiroz de Figueiredo, Patrícia Maria Guedes Paiva, Thiago Henrique Napoleão,

Tópico(s)

Plant-Microbe Interactions and Immunity

Resumo

This work describes the isolation of a lectin (CasuL) from the leaf pinnulae of Calliandra surinamensis and the evaluation of its cytotoxic, antimicrobial and antibiofilm properties. Proteins from pinnulae extract were precipitated with ammonium sulphate (60% saturation) and submitted to Sephadex G-75 chromatography, which yielded isolated CasuL (purification factor: 113). Native CasuL is an acidic protein (pI 5.82) with a relative molecular mass of 48 kDa. This lectin is also an oligomeric protein composed of three subunits and mass spectrometry revealed similarities with a Sorghum bicolor protein. CasuL did not undergo unfolding when heated but changes in conformation and hemagglutinating activity were detected at basic pH. CasuL did not reduce the viability of human peripheral blood mononuclear cells but was toxic to leukemic K562 cells (IC50 67.04 ± 5.78 μg/mL) and breast cancer T47D cells (IC50: 58.75 ± 2.5 μg/ mL). CasuL (6.25–800 μg/mL) only showed bacteriostatic effect but was able to reduce biofilm formation by Staphylococcus saprophyticcus and Staphylococcus aureus (non-resistant and oxacillin-resistant isolates). CasuL showed antifungal activity against Candida krusei causing alterations in cell morphology and damage to cell wall. In conclusion, the pinnulae of C. surinamensis leaves contain a thermo-stable lectin with biotechnological potential as cytotoxic, antibiofilm, and antifungal agent.

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