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Potential anti-inflammatory, antioxidant and antimicrobial activities of Sambucus australis

2017; Taylor & Francis; Volume: 55; Issue: 1 Linguagem: Inglês

10.1080/13880209.2017.1285324

1744-5116

Jhéssica Benevides Bahiense, Franciane Martins Marques, Mariana Moreira Figueira, Thais Souza Vargas, Tamara P. Kondratyuk, Denise Coutinho Endringer, Rodrigo Scherer, Márcio Fronza,

Natural product bioactivities and synthesis

Sambucus australis Cham. & Schltdl. (Adoxaceae) is used in Brazilian folk medicine to treat inflammatory disorders.To evaluate the in vitro anti-inflammatory, antioxidant and antimicrobial properties of S. australis.The anti-inflammatory activity of ethanol extracts of the leaf and bark of S. australis (1-100 μg/mL) were studied in lipopolysaccharide/interferon γ stimulated murine macrophages RAW 264.7 cells (24 h incubation) by investigating the release of nitric oxide (NO) and tumour necrosis factor-alpha (TNF-α) and in the TNF-α-induced nuclear factor kappa (NF-κB) assay. Minimum inhibitory concentration (MIC) was determined by the microdilution test (24 h incubation). Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and the NO scavenging assays. Chemical composition was assessed by LC-MS/MS.Antioxidant activities in the DPPH (IC50 43.5 and 66.2 μg/mL), FRAP (IC50 312.6 and 568.3 μg/mL) and NO radical scavenging assays (IC50 285.0 and 972.6 μg/mL) were observed in the leaf and bark ethanol extracts, respectively. Solely the leaf extract showed significant inhibition of NO and TNF-α production in RAW264.7 cells at concentrations of 2 and 100 μg/mL, respectively, and suppression of TNF-α inhibition of NF-κB by 12.8 and 20.4% at concentrations of 50 and 100 μg/mL, respectively. The extract also exhibited antibacterial activity against Salmonella typhimurium (MIC 250 μg/mL) and Klebsiella pneumoniae (MIC 250 μg/mL). LC-MS/MS revealed the presence of chlorogenic acid and rutin as major compounds.The results indicate that the ethanol leaf extract of S. australis exhibit prominent anti-inflammatory effects.