Produção Nacional
Revisado por pares
Polypyridyl Ruthenium Complexes: Novel DNA-Intercalating Agents against Human Breast Tumor
2017; Brazilian Chemical Society; Linguagem: Inglês
10.21577/0103-5053.20170019
ISSN1678-4790
AutoresJoão P. Barolli, Rodrigo S. Corrêa, Fábio S. Miranda, Juliana Silva Ribeiro, Carlos Bloch, Javier Ellena, Virtudes Moreno, Márcia R. Cominetti, Alzir A. Batista,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoThis paper describes a new series of four DNA-intercalating agents with promising anticancer activities, based on ruthenium(II) with the planar ligand dpqQX(dppb = 1,4-bis(diphenylphosphine)butane and PPh 3 = triphenylphosphine) were characterized by 31 P{ 1 H} nuclear magnetic resonance (NMR) and infrared spectroscopies, cyclic voltammetry, molar conductance measurements, elemental analysis, mass spectrometry and X-ray diffraction analysis for complex ct-[RuCl 2 (PPh 3 ) 2 (dpqQX)].Their in vitro cytotoxic activities against MDA-MB-213 and MCF-7 breast cancer cells were evaluated and compared with normal L-929 cells.Low drug concentration at which 50% of the cells are viable relative to the control (IC 50 ) values were obtained for all four complexes compared with a reference metallodrug, cisplatin.In addition, DNA affinity studies from titrations, as well as the images obtained by atomic force microscopy (AFM) involving pBR322 plasmid DNA, suggest interactions between the metal complexes and the DNA macromolecule, in which they act as intercalating agents.The intercalation of the complexes with DNA was confirmed by viscosity measurements.
Referência(s)