Solid-Phase Peptide Synthesis
1976; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-12-516302-6.50008-6
AutoresBruce W. Erickson, R. B. Merrifield,
Tópico(s)Biochemical and Structural Characterization
ResumoPeptides are a very important biologically active class of naturally occurring compounds, and the number of newly discovered peptides has grown rapidly. The need for a large number of synthetic peptides and peptide analogs has grown even faster, and the most effective preparative methods are essential. This chapter describes solid-phase peptide synthesis. In the original and simplest form, solid-phase peptide synthesis involves a heterogeneous reaction mixture composed of an insoluble resin-bound peptide chain, a soluble activated amino acid derivative, and a solvent. The solid-phase principle for peptide synthesis was conceived and developed for providing a rapid, simplified, and effective way to prepare peptides and small proteins. This chapter focuses on solid supports, methods for attachment and removal of the peptide chain, protecting group schemes, and coupling methods. It also discusses difficult couplings and peptide ligation methods. The chapter also describes special synthetic applications including protein–nucleic acid compounds and the de novo design and synthesis of peptides and proteins.
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