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2D and 3D cell cultures – a comparison of different types of cancer cell cultures

2016; Termedia Publishing House; Linguagem: Inglês

10.5114/aoms.2016.63743

1896-9151

Marta Kapałczyńska, Tomasz Kolenda, Weronika Przybyła, M Zajaczkowska, Anna Teresiak, Violetta Filas, Matthew Ibbs, Renata Bliźniak, Łukasz Łuczewski, Katarzyna Lamperska,

Viral Infectious Diseases and Gene Expression in Insects

Cell culture is a widely used in vitro tool for improving our understanding of cell biology, tissue morphology, and mechanisms of diseases, drug action, protein production and the development of tissue engineering. Most research regarding cancer biology is based on experiments using two-dimensional (2D) cell cultures in vitro. However, 2D cultures have many limitations, such as the disturbance of interactions between the cellular and extracellular environments, changes in cell morphology, polarity, and method of division. These disadvantages led to the creation of models which are more closely able to mimic conditions in vivo. One such method is three-dimensional culture (3D). Optimisation of the culture conditions may allow for a better understanding of cancer biology and facilitate the study of biomarkers and targeting therapies. In this review, we compare 2D and 3D cultures in vitro as well as different versions of 3D cultures.