Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
2017; Nature Portfolio; Volume: 14; Issue: 4 Linguagem: Inglês
10.1038/nmeth.4179
ISSN1548-7105
AutoresTodd M. Gierahn, Marc H. Wadsworth, Travis K. Hughes, Bryan D. Bryson, Andrew Butler, Rahul Satija, Sarah M. Fortune, J. Christopher Love, Alex K. Shalek,
Tópico(s)RNA and protein synthesis mechanisms
ResumoSingle-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.
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