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Evaluation of vascular tone and cardiac contractility in response to silver nanoparticles, using Langendorff rat heart preparation

2017; Elsevier BV; Volume: 13; Issue: 4 Linguagem: Inglês

10.1016/j.nano.2017.01.017

1549-9642

Manuel Alejandro Ramirez-Lee, Pedro Pablo Martinez-Cuevas, Héctor Rosas-Hernández, Cuauhtémoc Oros‐Ovalle, Mariela Bravo-Sánchez, Gabriel Alejandro Martínez-Castañón, Carmen González,

Hydrogen's biological and therapeutic effects

Silver nanoparticles (AgNPs) have been widely used because of their antimicrobial properties. However, several reports suggest that AgNPs exposure promote cardiac effects that involve nitric oxide (NO) and oxidative stress (OS). Nevertheless, there are no studies related to AgNPs-induced effects in cardiac physiology. The aim of this study was to evaluate the AgNPs direct actions on coronary vascular tone and cardiac contractility using Langendorff rat heart preparation. Low concentrations of AgNPs (0.1 and 1 μg/mL) increased NO derived from inducible NO-synthase (iNOS), without modifying cardiac parameters. Meanwhile, high concentrations (10 and 100 μg/mL) promoted a sustained vasoconstriction and increased cardiac contractility related to OS, leading to rhabdomyolysis. Furthermore, AgNPs were internalized in the cardiac muscle, hindering classic actions induced by phenylephrine (Phe) and acetylcholine (ACh). These data suggest that AgNPs affect cardiac physiology in function of the concentration and in part of the NO generation, NOS expression and OS.