Produção Nacional
Revisado por pares
Vitamin E nanoemulsion activity on stored red blood cells
2017; Wiley; Volume: 27; Issue: 3 Linguagem: Inglês
10.1111/tme.12394
ISSN1365-3148
AutoresC. A. L. Silva, Clayton A. Azevedo Filho, Goreti Pereira, D. C. N. Silva, Maria Carolina Accioly Brelaz de Castro, A. F. Almeida, Sheyla C. Lucena, Beate S. Santos, Maria Lourdes Barjas‐Castro, Adriana Fontes,
Tópico(s)Erythropoietin and Anemia Treatment
ResumoSUMMARY Background Stored red blood cells (RBCs) undergo numerous changes that have been termed RBC storage lesion, which can be related to oxidative damage. Vitamin E is an important antioxidant, acting on cell lipids. Thus, this study aimed to investigate vitamin E activity on stored RBCs. Methods We prepared a vitamin E nanoemulsion that was added to RBC units and stored at 4 °C. Controls, without vitamin E, were kept under the same conditions. Reactive oxygen species (ROS) production was monitored for up to 35 days of storage. RBC elasticity was also evaluated using an optical tweezer system. Results Vitamin E‐treated samples presented a significant decrease in ROS production. Additionally, the elastic constant for vitamin E‐treated RBCs did not differ from the control. Conclusion Vitamin E decreased the amount of ROS in stored RBCs. Because vitamin E acts on lipid oxidation, results suggest that protein oxidation should also be considered a key factor for erythrocyte elastic properties. Thus, further studies combining vitamin E with protein antioxidants deserve attention, aiming to better preserve overall stored RBC properties.
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