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Microbial diversity of a full‐scale UASB reactor applied to poultry slaughterhouse wastewater treatment: integration of 16S rRNA gene amplicon and shotgun metagenomic sequencing

2017; Wiley; Volume: 6; Issue: 3 Linguagem: Inglês

10.1002/mbo3.443

2045-8827

Tiago Palladino Delforno, Gileno Vieira Lacerda-Júnior, Melline Fontes Noronha, Isabel Kimiko Sakamoto, Maria Bernadete Amâncio Varesche, Valéria Maia de Oliveira,

Bacteriophages and microbial interactions

Abstract The 16S r RNA gene amplicon and whole‐genome shotgun metagenomic ( WGSM ) sequencing approaches were used to investigate wide‐spectrum profiles of microbial composition and metabolic diversity from a full‐scale UASB reactor applied to poultry slaughterhouse wastewater treatment. The data were generated by using MiSeq 2 × 250 bp and HiSeq 2 × 150 bp Illumina sequencing platforms for 16S amplicon and WGSM sequencing, respectively. Each approach revealed a distinct microbial community profile, with Pseudomonas and Psychrobacter as predominant genus for the WGSM dataset and Clostridium and Methanosaeta for the 16S r RNA gene amplicon dataset. The virome characterization revealed the presence of two viral families with Bacteria and Archaea as host, Myoviridae, and Siphoviridae . A wide functional diversity was found with predominance of genes involved in the metabolism of acetone, butanol, and ethanol synthesis; and one‐carbon metabolism ( e.g., methanogenesis). Genes related to the acetotrophic methanogenesis pathways were more abundant than methylotrophic and hydrogenotrophic, corroborating the taxonomic results that showed the prevalence of the acetotrophic genus Methanosaeta . Moreover, the dataset indicated a variety of metabolic genes involved in sulfur, nitrogen, iron, and phosphorus cycles, with many genera able to act in all cycles. BLAST analysis against Antibiotic Resistance Genes Database ( ARDB ) revealed that microbial community contained 43 different types of antibiotic resistance genes, some of them were associated with growth chicken promotion ( e.g., bacitracin, tetracycline, and polymyxin).