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Supercritical fluid extraction of polyphenols from grape seed ( Vitis vinifera ): Study on process variables and kinetics

2017; Elsevier BV; Volume: 130; Linguagem: Inglês

10.1016/j.supflu.2017.02.013

1872-8162

Carla Da Porto, Andrea Natolino,

Phytochemicals and Antioxidant Activities

Polyphenol extraction from white grape seeds using supercritical carbon dioxide (SC-CO2) containing different percentages of ethanol-water mixture (57% v/v) as co-solvent was carried out at 40 °C. Box–Behnken experimental design was used to optimize the process variables: pressure (80, 100, 120 bar), CO2 flow rate (2, 4 and 6 kg/h) and co-solvent percentages (10, 15, and 20% (w/w)). Total polyphenols (TPC) and fractionation of proanthocyanidins were determined.The statistically generated optimum extraction conditions to obtain the highest total polyphenol concentration were a pressure of 80 bar, CO2 flow rate of 6 kg/h and 20% (w/w) co-solvent. The value achieved in validation experimental was 7132 mg GAE/100 g DM. Under these operating conditions a good extraction of FI (>1000 mg catechin/100 g DM) and FII (>800 mg catechin/100 g DM) are obtained.